两步PCR法检测鸡新城疫疫苗中鸡毒支原体污染的研究  

Study on the Detection of MG Contamination in Live Vaccines by Two-StepPCR

作　　者：贾丽艳[1] 逯晓敏[2] 张映[2] 

机构地区：[1]山西农业大学食品科学与工程学院,山西太谷030801 [2]山西农业大学动物科技学院,山西太谷030801

出　　处：《山西农业大学学报（自然科学版）》2008年第3期316-319,共4页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：山西省教委科技项目(99015)

摘　　要：本试验以Genbank中登录的鸡毒支原体的16s rRNA基因序列为标准,根据前人设计的两套引物(外引物和内引物)对鸡毒支原体DNA进行两次PCR扩增,建立两次PCR扩增体系和条件。最后,用此体系和条件对从市场上随机购买的17支鸡新城疫La Sota系活疫苗样品和13支鸡新城疫Ⅰ系活疫苗样品进行检测。结果显示,运用建立的两次PCR扩增体系和条件可以从疫苗样品中检测到支原体的污染,外引物的检出率为23.3%(7/30),内引物的检出率为10%(3/30)。In this experiment, the system and condition of two-step-PCR to detect MG contamination were established with two pairs of primers which had been designed by the predecessors according to the 16s rRNA gene sequence of MG published in Genbank. This method was applied to detect the samples including13 batches of newcastle disease live vaccines. The results showed that the MG contamination can be detected in the live vaccines by the two-step-PCR. The rate of detection by the first PCR and the second were 23.3% （7/30） and 10% （3/30）, respectively.

关 键 词：支原体 疫苗 检测 PCR 

分 类 号：Q781[生物学—分子生物学]