香根鸢尾挥发油的化学成分分析及抗菌活性研究  

Chemical Composition and Antimicrobial Activity of the Essential Oil of Iris pallida Lam.

作　　者：邓国宾[1] 张晓龙 王燕云 林瑜 陈小兰[1] 

机构地区：[1]云南大学科学学院,云南昆明650091 [2]云南瑞升烟草技术(集团)有限公司,云南昆明650106 [3]昆明爱德组培有限公司,云南昆明650031

出　　处：《林产化学与工业》2008年第3期39-44,共6页Chemistry and Industry of Forest Products

基　　金：国家烟草专卖局项目(110200601032);云南省科委项目(2006GG24)

摘　　要：采用GC-MS技术分析了新鲜的和自然陈化的鸢尾挥发油的化学组成,确定了各种成分的化学结构与GC含量,并采用滤纸片琼脂平板扩散法与微量肉汤稀释法针对3种真菌和12种细菌(包括7种临床致病菌)进行了挥发油的药敏实验。结果表明:新鲜鸢尾挥发油共鉴定出26种成分,占总峰面积的92.09%,由醛类、酮类、酸类、酯类等化合物组成,其中十四酸(31.77%)、己酸(15.33%)、3-甲基丁酸(12.20%)、戊酸(9.29%)、庚酸(7.91%)、3-甲基戊酸(7.25%)、辛酸(2.44%)、2,4′-二羟基-3′-甲基苯乙酮(1.04%)是主要成分;自然陈化3年鸢尾挥发油共鉴定出45种成分,占总峰面积的85.63%,主要由醛类、酮类、酸类、酯类、醇类、酚类等化合物组成,其中十四酸(28.02%)、十四酸乙酯(10.42%)、(Z,Z)-9,12-十八碳二烯酸(7.67%)、十二酸(6.41%)、己酸(5.14%)、十六酸(4.44%)、十二酸乙酯(4.09%)、亚油酸乙酯(3.87%)、十六酸乙酯(3.13%)、油酸乙酯(2.85%)、6-甲基-α-紫罗兰酮(1.39%)、n-癸酸(1.31%)是主要成分;新鲜鸢尾挥发油对大部分微生物均具有很好的抗菌作用,其最低抑菌浓度(MIC)为0.25g/L,最低杀菌浓度(MBC)为0.35g/L;自然陈化3年鸢尾挥发油对大部分微生物均具有很好的抗菌作用,其MIC为0.05g/L,MBC为0.08g/L。Essential oils from the fresh rhizomes and the naturally aged rhizomes of Iris paUida Lam. were analyed by GC-MS, respetively, and the relative contents of constituents were determined by area normalization method. The antimierobial activity of the essential oil was evaluated against 3 fungi and 12 bacteria including 7 clinical isolated strains. The results show that： twentysix volatile components were identified and determined in the oil of the fresh rhizomes, accounting for 92.09 % of the total area of the peak, and the main compounds of the oil were tetradeeanoie acid （31.77 % ）, hexanoie acid（ 15.33 % ）, 3-methyl-butanoie acid（ 12.20 % ）, pentanoic acid （ 9.29 % ） , heptanoie acid （ 7.91% ）, 3-methyl pentanoie acid （ 7.25 % ） , oetanoie acid （2.44 % ）,2＇ ,4＇-dihydroxy-3＇-methylaeetophenone（ 1.04 % ）. Forty-five volatile components were identified and determined in the oil of the naturally aged rhizomes, accounting for 85.63 % of the total area of the peak, and the main compounds of the oil were tetradeeanoie acid（ 28.02 % ） , tetradeeanoie acid, ethyl ester（ 10.42 % ） , 9,12-oetadeeadienoie acid （7.67 % ）, dodecanoie acid（6.41% ） , hexanoie acid（5.14 % ） , hexadeeanoie acid（4.44 % ） , dodeeanoie acid ethyl ester（4.09 % ） , linoleie acid ethyl ester（3.87 % ） , hexadeeanoie acid ethyl ester（ 3.13 % ） , ethyl oleate （2.85 % ） , α-ionone, 6-methyl-（ 1.39 % ） , and deeanoie acid （ 1.31% ） ; the oil of the fresh rhizomes presented a broad antimierobial spectrum, and the minimal inhibitory concentration （MIC） value was 0.25 g/L and the minimal bactericidal concentration （MBC） value was 0.35 g/L; the oil of the naturally aged rhizomes presented a broad antimierobial spectrum, and had better antimicrobial activity against gram-positive bac- teria, MIC value was O. 05 g/L and MBC value was 0. 08 g/L.

关 键 词：香根鸢尾 挥发油 抗菌 

分 类 号：TQ424.19[化学工程] O532.23[理学—等离子体物理]