汉、回、蒙古族MBL基因Exon Ⅰ 54位密码子点突变频率及血浆含量相关性研究  

The Correlation between Point Mutation of MBL Exon Ⅰ and Its Plasma Concentration in North Han,Hui and Mongolia People

作　　者：李萍[1] 韩瑞[1] 程建君[1] 贾天军[1] 张庶民[2] 

机构地区：[1]河北北方学院医学技术学院检验系,河北张家口075000 [2]中国药品生物制品检定所,北京100000

出　　处：《河北北方学院学报（医学版）》2008年第3期1-3,共3页Journal of Hebei North University:Medical Edition

基　　金：河北省自然科学基金资助项目(编号:C2006000875);河北省科学技术研究与发展指导计划项目(编号:072761197)

摘　　要：目的:初步调查我国北方地区汉、回、蒙古族健康人群MBL基因ExonⅠ54位密码子点突变的情况,检测其血浆MBL含量,探讨二者的相关性。方法:用PCR-RFLP检测MBL基因点突变频率;用MBLOli-gomerELISA试剂盒测定血浆MBL含量。结果:健康汉族人该基因突变频率0.23,血浆MBL含量均值1.99±1.50mg/L,二者负相关(r=-0.62);回族人突变频率0.15,MBL含量均值3.40±2.55mg/L,二者负相关(r=-0.67);蒙古族人基因突变频率0.18,含量均值2.52±1.95mg/L,二者负相关(r=-0.64)。结论:健康汉、回、蒙古族人MBL基因54位密码子点突变频率与其血浆含量均呈负相关。Objective： To study and analyze the point mutation at code 54 in healthy North Han, Hui and Mongolia people, measure the plasma levels of MBL, and analyze the association between gene mutation frquencies and plasma MBL concentrations. Methods： PCR-RFLP was used to detect MBL point mutation; The MBL plasma concentrations were measured by MBLOliger ELISA kit. Results：Frequencies of point mutation at code 54 of MBL coding gene in healthy Han, Hui and Mongolia people were 0.23,0.15,0. 18. The plasma MBL concentrations were 1.99±1.50mg/L, 3. 40±2. 55mg/L and 2.52±1.95mg/L. There was negtive correlation between MBL concentrations and gene mutation frequencies in Han, Huis and Mongolia people（r=- 0.62, r= -0. 67 ,r= -0. 64）. Conclusion：The relationship between mutation frequency at code 54 of MBL gene and the plasma MBL concentrations in North Han, Huis and Mongolia people is negative correlation.

关 键 词：汉族 回族 蒙古族 基因顺序 

分 类 号：R318[医药卫生—生物医学工程]