毒死蜱完全抗原的合成与表征  

Synthesis and Characterization of Artificial Antigen for the Organophosphorus Chlorpyrifos

作　　者：冯春梁[1] 张美容[1] 陈建芳[1] 孙越[1] 

机构地区：[1]辽宁师范大学化学化工学院功能材料化学研究所,大连116029

出　　处：《世界科技研究与发展》2008年第2期126-128,共3页World Sci-Tech R&D

基　　金：国家自然科学基金资助项目(60572009);辽宁省科技攻关计划项目(2005220024);辽宁省自然科学基金项目(2040686)

摘　　要：在碱性条件下将毒死蜱原药与3-巯基丙酸反应,得到毒死蜱半抗原(O,O二乙基-O-[3,5-二氯-6-(2-羧乙基)硫代-2-吡啶基]硫代磷酸酯),采用活泼酯法将生成的半抗原与牛血清白蛋白(BSA)偶联,制备完全抗原。红外光谱、气相色谱-质谱联用仪及紫外光谱测试结果表明,所合成的物质均为目标产物。本方法简便易行,为利用免疫传感器快速检测有机磷农药毒死蜱残留量解决了一个关键技术问题。In this paper ,the hapten of the organophosphorus insecticide chlorpyrifos,o,o-diethyl-o-[3,5-dichloro-6-（2-carboxyethyl）thio-2-pyridyl]phosphorothioate was synthesized by using technicalgrade chlorpyrifos reacted with 3-marcapropanoic acid in the hot alkazid solution and its structure was confirmed by IR and GC/MS. The hapten was conjugated to bovine serum albumin （BSA） with the active ester method to form artificial immune antigen. The artificial immune antigen was identified by ultraviolet spectrophotometry and IR spectrum . The results showed that the artificial immune antigen of chlorpyrifos was synthesized successfully. This method made it possible to establish immunoassay of chlorpyrifos.

关 键 词：毒死蜱 完全抗原 活泼酯法 牛血清白蛋白 3-巯基丙酸 

分 类 号：TQ450.1[化学工程—农药化工]