169例急性白血病免疫表型分析  

Immunophenotype Analysis on 169 Cases of Acute Leukemia

作　　者：李艳明[1] 曹志红[1] 彭敏源[1] 

机构地区：[1]中南大学湘雅医院,湖南长沙410008

出　　处：《实用预防医学》2008年第3期668-671,共4页Practical Preventive Medicine

摘　　要：目的探讨急性白血病的免疫表型特征,为急性白血病的诊断提供依据。方法收集169例初治急性白血病患者骨髓或外周血标本,采用流式细胞仪对其白血病细胞进行免疫表型分析。结果95例急性髓细胞白血病(acute myeloblastic leukemia,AML)患者中59例为纯髓系抗原表达,34例伴淋系抗原(CD19、CD7)表达。64例急性淋巴细胞白血病(acute lymphoblastic leukemia)患者中62例表达淋系抗原,纯T系抗原表达8例,纯B系抗原表达44例,6例伴有髓系抗原(CD33)表达;1例ALL-L2为纯髓系(CD33、CD34)表达,1例ALL-L2八个抗原检测均为阴性。10例急性混合型白血病(mixedacute leukemia,MAL)同时表达两种以上淋系和髓系抗原标志。结论免疫分型能助诊一些特殊类型的白血病(如minimally differentiated acute myeloid leukemia,AML-M0;MAL);免疫分型是对白血病细胞形态学诊断的重要补充。Objective To explore the immunophenotypical characteristics for the diagnosis of acute leukemia. Method The immunophenotype of bone marrow or blood samples collected from 169 patients with newly diagnosed acute leukemia was analyzed by flow cytometer. Results Myeloid antigens were expressed alone in 59 of 95 patients with acute myeloid leukemia （ AML ）, 34 patients with AML showed aberrant lymphoid antigens （ CD19 or CD7 ） expression. Lymphoid antigens were expressed in 62- of 64 patients with acute lymphoid leukemia （ALL）, and T or B lymphoid antigens were detected alone in 8 or 44 patients respectively. Six patients with ALL showed aberrant myeloid antigens 0D33 expression. In one patient with ALL- L2, only 0D33 and 0D34 were detectable and in another patient with ALL- L2 eight antigens were undetectable. Ten patients with mixed acute leukemia （MAL） showed lymphoid and myeloid antigens more than two kinds. Conclusion To some leukemia （ ie. AML - M0 or MAL ）, immunophenotype has diagnostic signification and it is the important complement to morphology.

关 键 词：免疫分型 急性白血病 分化簇 流式细胞仪 

分 类 号：R557[医药卫生—血液循环系统疾病]