凝血混合质控血浆的制备、评价和应用  

Preparation,Evaluation and Application of Mixed Coagulation Control Plasma

作　　者：余启华[1] 王孝华[1] 易建文[1] 佘鸥[2] 欧阳旋[1] 

机构地区：[1]湖南省第二人民医院检验科,湖南长沙410007 [2]湖南省临床检验中心

出　　处：《实用预防医学》2008年第3期880-882,共3页Practical Preventive Medicine

摘　　要：目的探讨凝血混合质控血浆的制备、瓶间差和稳定性及其在室内质量控制(IQC)中的应用。方法制备凝血混合质控血浆,-20℃冰箱保存,选用体检组混合血浆进行瓶间差的测定,同时用Thrombolyzer Compact-X血凝分析仪上对体检组和患者组混合血浆进行稳定性检测以及常规室内质量控制的开展。结果混合血浆的分装引起的瓶间差很小。体检组混合血浆在-20℃冰箱保存120d内PT-s、APTT、Fib稳定性良好,患者组混合血浆的PT-s、APTT测定值有增加的趋势,Fib降低。患者组混合血浆在90d后三个检测项的偏倚(Bias%)虽然可以接受,但变异系数较大,不再适合作为室内质控物使用。结论凝血混合血浆在一定时间内能够满足临床实验室在单值水平上室内质量控制的使用要求。Objective To observe the preparation, the vial - to- vial variability, the stability of the mixed coagulation control plasma, and the application in internal quality control （1（2（3）. Methods The mixed coagulation control plasma was prepared to store at - 20 13 condition. The vial - to- vial variability was determined with the mixed coagulation control plasma of volunteers, the stability of the plasma of the patients and the volunteers was determined with thrombolyzer compact- X instrument, which was applied to internal quality control（IQC）. Results The vial- to- vial variability of the mixed coagulation control plasma was little. The values ol PT - s, APTT and Fib of the mixed coagulation control plasma of volunteers were stable within 120 days, while the values of PT- s and APTT of the patients were increased and Fib was decreased. Bias% of the patient group can be accepted when to be stored at - 20 13 condition after 90 days, but CV% was not ideal, and the patient plasma can not be applied to internal quality control（IQC）. Conclusions The mixed coagulation control plasma can suit the requirements of internal quality control （IQC） to a certain extent.

关 键 词：凝血混合质控血浆 瓶间差 稳定性 室内质量控制(IQC) 

分 类 号：R457[医药卫生—治疗学]