马桑内酯激活的星形胶质细胞条件培养液对大鼠脑内谷氨酸及其受体GluR2表达的影响  

Effects of coriaria lactone-activated astrocyte conditioned medium on the expressions of glutamate and GIuR2 in the rat brain

作　　者：张树华[1] 朱长庚[2] 刘庆莹[2] 魏瑛[2] 王伟[3] 

机构地区：[1]滨州医学院病理学教研室,山东省滨州市256603 [2]华中科技大学同济医学院神经科学研究所,武汉430030 [3]华中科技大学同济医学院神经内科,武汉430030

出　　处：《中华神经医学杂志》2008年第6期549-553,共5页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金（30230140）

摘　　要：目的揭示马桑内酯（CL）激活的星形胶质细胞（Ast）条件培养液（ACM）对大鼠脑内谷氨酸（Glu）及其受体GluR2表达的影响。方法取成年健康雄性SD大鼠48只，采用随机数字表法分为对照组（16只）和CL组（32只），对照组侧脑室注射未加任何刺激物的ACM10μL，CL组侧脑室注射CL激活的ACM10uL；按注射后取材时问不同又分为2h、4h、8h和12h四个亚组，对照组每亚组4只．CL组每亚组8只。观察两组大鼠的行为表现，用免疫组化、免疫荧光检测脑内G1u和GluR2表达的变化，Western blot检测脑内GluR2含量的变化。结果CL组大鼠有痫样发作，而对照组无痫样发作；免疫组化和免疫荧光检测结果显示，CL组皮质和海马区Glu表达较对照组显著增强，4h时差异有统计学意义伊〈0．05），而CL组皮质和海马区GluR2的表达较对照组弱，4h时差异有统计学意义f氏0．05）。Westem blot结果显示，CL组4个时间点的GluR2表达均较对照组含量显著降低，差异有统计学意义（氏0．05）。结论CL激活的ACM能显著增强脑内神经元G1u的表达，降低GluR2的表达，进而诱发癫痫。Objective To explore the effects of coriaria lactone （CL）-activated astrocytes （Ast） conditioned medium （ACM） on the expressions of glutamate （Glu） and GluR2 in the brain of rat. Methods Asts of hippocampus were cultured according to the McCarthy and DeVellis＇s method, and then the ACM was collected. Forty-eight male adult Sprague-Dawley （SD） rats were randomly divided into the control group （n=16） and the CL group （n=32）. Rats in the control group were administered 10 μL ACM i. c. v., which was not added any stimulating substance. Rats of the CL group were injected i. c. v.10 μL CL-activated ACM. The rats in both groups were subdivided into post-injection 2, 4, 8, 12 h subgroups, 4 in each subgroup in the control group and 8 in each subgroup in the CL group. The behaviors of the rats were observed and the expressions of Glu and GluR2 in the cerebral cortex and hippocampus were detected with immunohistochemistry and immunofluorescence. The content of GluR2 was tested with Western blot. Results The rats injected with CL-activated ACM showed seizure activities, whereas the rats of the control group showed no seizure activities. The expression of Glu in cerebral cortex and hippocampus in the brains injected with CL-activated ACM was increased compared with the control group at 4 h （P〈0.05）, but the expression of GluR2 was attenuated compared with the control group at 4 h（P〈0.05）. The results of GluR2 in the cerebral cortex and hippocampus detected with Western blot were different significantly with control group（P〈0.05）. Conclusion CL-activated ACM can enhance the expression of Glu and reduce the expression of GluR2 in the brain of rat, resulting in the activation of AMPA pathway and the Ca2＋ influx, and then induce seizure activities.

关 键 词：星形胶质细胞 条件培养液 马桑内酯 谷氨酸 受体 

分 类 号：R285[医药卫生—中药学]