长穗桑四倍体诱导初步研究  

Preliminary Study on Tetraploid Induction of Morus wittiorum

作　　者：武宇坤[1] 何建[1] 王西木[1] 郭启高[1] 梁国鲁[1] 

机构地区：[1]西南大学园艺园林学院,重庆400716

出　　处：《热带农业科技》2008年第2期26-27,41,共3页Tropical Agricultural Science & Technology

摘　　要：以长穗桑为试验材料,对其进行继代增殖及对最佳增殖培养基进行了筛选,并用组织培养技术结合秋水仙素溶液浸泡法以获得四倍体新材料。结果表明:增殖培养基采用MS+BA2.0mg/L+NAA0.2mg/L+蔗糖30mg/L最佳,其最高的增殖系数为3.2。四倍体诱导率达到26.7%,其染色体数目为2n=4x=56,最佳诱导条件为用0.2%的秋水仙素溶液浸泡3d。所获得的四倍体最适生根培养基为1/2MS+BA0.1mg/L+NAA0.05mg/L+30mg/L蔗糖,生根率达到100%。An experiment ofMorus wittiorum was carried out in vitro with colchicine solutions to select the favourable proliferation medium for tewaploid. The results indicated that the optimum proliferation medium was MS＋BA2.0mg/L＋NAA0,2mg/L＋sugar 30mg/L. The proliferation coefficient could reach to 3.2 and induction rate of tetraploid was 26,7% with chromosome number of 2n=4x=56. The optimal induction result was obtained by treatment with 0.2% colchicine solution for 3 days. 1/2MS＋BA0.1 mg/L＋NAA0.05mg/L＋sugar 30mg/L was optimal for rooting, and rooting rate reached 100%.

关 键 词：长穗桑 多倍体 组织培养 诱导 

分 类 号：S663.9[农业科学—果树学]