不同产地新疆赤芍中芍药苷的含量测定  

Determination of Paeoniflorin in Radix Paeonia hybrida from Different Habitats

作　　者：杨媛媛[1] 马晓康 顾政一 周钢 王宇卿[1] 

机构地区：[1]石河子大学药学院新疆特种植物药资源省部共建教育重点实验室,新疆石河子832002 [2]新疆维吾尔自治区药品检验所,乌鲁木齐830002 [3]新疆药物研究所,乌鲁木齐830004

出　　处：《医药导报》2008年第7期763-764,共2页Herald of Medicine

基　　金：新疆维吾尔自治区重点实验室基金资助项目(基金编号:XJYS-0207-2006-04)

摘　　要：目的测定10个不同产地新疆赤芍药材中芍药苷含量。方法采用Agilent TC-C18色谱柱(4.6mm×150mm,5μm);流动相为甲醇-0.05mol·L-1磷酸二氢钾溶液(34.8:65.2);检测波长:230nm;流速1mL·min-1;进样量10μL。结果芍药苷线性范围为0.1002～1.0020mg,r=0.9999(n=6);平均加样回收率为98.8%～101.5%,RSD为0.355%～1.770%(n=9)。结论该实验准确测定10个不同产地新疆赤芍中芍药苷的含量,10批药材中芍药苷的含量均符合《中华人民共和国药典》标准。Objective To determine the contents of paeoniflorin in Radix Paeonia hybrida from 10 different habitats. Methods An Agilent TC-C18 column （4. 6 mm × 150 mm,5 μm） was used with the mobile phase being methanol-0. 05 mol · L^-1 KH2PO4 solution （34.8 ： 65.2, V/V,flow rate at 1.0 mL · min^-1 ,detecting wavelength as 230 nm, and the loading volume as 10 μL. Results The linear range was 0.022 4 - 0. 448 0 mg （ r = 0. 999 9, n = 6 ）, and the average recocery of paeoniflorin was 98.8% - 101.5%, with RSD as 0. 355% -1. 770% （n = 9）. Conclusion Paeoniflorin from plants in 10 different habitats were accurately determined, and which all meet up the pharmacopoeia standards.

关 键 词：新疆赤芍 芍药苷 色谱法 高效液相 

分 类 号：R828.71[医药卫生—临床医学] R927.11