鹅副粘病毒QY分离株的蚀斑纯化及F基因的克隆与序列分析  

Cloning and sequencing of F gene of avian paramyxovirus strain QY isolated from goose that were purified by plaque-purification test

作　　者：王友令[1] 李福伟[1] 秦卓明[1] 

机构地区：[1]山东省农业科学院家禽研究所,山东济南250100

出　　处：《浙江农业学报》2008年第3期158-162,共5页Acta Agriculturae Zhejiangensis

基　　金：山东省科技攻关重点支持项目(030317);山东省自然基金支持项目(031020101)

摘　　要：将鹅副粘病毒QY分离株蚀斑纯化后,根据已发表的鹅副粘病毒SF02株全基因序列,分别设计合成3对引物,设计合成F基因引物,经RT-PCR方法扩增,克隆入PMD18-T载体,鉴定、测序。测序后拼接得出F基因的全序列长度为1 662 kb,编码553个氨基酸残基,与GenBank下载的几株参考毒株比较F基因编码区的核苷酸序列,发现所测QY株F基因核苷酸序列同源性与参考毒株SF02为98.3%,与LaSota分别为82.4%,同源性分析表明分离株QY与国内的强毒株参考毒株的同源性较高。After avian paramyxovims isolated strain QY from goose were purified by plaque-purification. According to the complete genome sequence of GMPV SF02 isolate published, five pair of primers were designed to amplify F gene by RTPCR,DNA product were cloned to PMD18-T vector, identified, sequenced. The results of sequencing showed that the nucleotide sequence of F gene was 1 662 bp in length and has a single open reading frame which encodes 553 amino acid residues. The results of sequence analysis indicated that some reference strains loaded down from GenBank and QY strain exhibited 98.3% and 82.4% homology, respectively, as compared with reference strain SF02 and strain LaSota. The QY strain homology was high similar to virulence strain of China.

关 键 词：鹅副粘病毒 蚀斑纯化 F基因 

分 类 号：S852.65[农业科学—基础兽医学]