荧光定量PCR联合ELISA检测丙型肝炎病毒的临床应用研究  

Detection of hepatitis C virus by FQ-PCR and ELISA: Clinical application

作　　者：高英英[1] 马雷[2] 孙玉鸿[1] 

机构地区：[1]佳木斯大学临床医学院检验系,黑龙江佳木斯154003 [2]佳木斯大学附属第一医院检验科,黑龙江佳木斯154003

出　　处：《黑龙江医药科学》2008年第3期17-18,共2页Heilongjiang Medicine and Pharmacy

摘　　要：目的:探讨荧光定量逆转录聚合酶链反应(FQ-PCR)、酶联免疫吸附试验(ELISA)联合检测丙型肝炎病毒的临床意义。方法:用FQ-PCR联合ELISA方法检测117例临床已确诊为丙型肝炎患者的血清,同时检测它们的丙氨酸氨基转移酶(AAT)。结果:1抗-HCV阳性率随着HCV-RNA含量的升高而增高,其阳性率与HCV-RNA含量呈正相关。287例HCV-RNA阳性标本中,有50例ALT异常,其异常率随着HCV-RNA含量的升高而增高,ALT异常率与HCV-RNA含量呈正相关,ALT水平和HCV-RNA含量之间呈正相关。3FQ-PCR检测敏感性为74.4%(87/117),漏检率为25.6%(30/117)。ELISA法检测敏感性为76.9%(90/117),漏检率为23.1%(27/117)。两种方法联合检测敏感性为92.3%(108/117),漏检率为7.7%(9/117)。结论:两种方法联合检测,互相补充,大大提高了丙型肝炎病毒的检出率,为临床早期,准确诊断丙型病毒肝炎、监测病情、观察疗效提供了有力的依据,为献血人员筛选和血制品安全提供了有力保障。Objective ： To discuss the clinical significance of detection of hepatitis C virus by FQ--PCR and ELISA. Methods：We detected of 117 sera of clinical patients who had been diagnosed as hepatitis C, and at the same time tested their atanine aminotransferase（AAT）. Results ：（1） Anti -- HCV＇s positive rate rose with HCV--RNA content increase. By using correlation analysis,anti--HCV positive rate and HCV-- RNA content showed positive correlation. （2）There were 50 AAT abnormal ones in 87 HCV--RNA positive samples. AAT abnormal rate rose with HCV--RNA content increase,showing positive correlation by using correlation analysis, and there was a positive correlation between the AAT level and HCV--RNA content. （3）FQ--PCR detection sensitivity was 74.4%（87/117）,and omission factor was 25.6%（30/117）. ELISA detection sensitivity was 76.9%（90/117）,and omission factor was 23. 1%（27/117）. Combined detection sensitivity of two kinds of methods was 92. 3%（108/117）, and omission factor was 7. 7%（9/117）. Combined detection sensitivity was higher than that of separate. Conclusion：The combined detection is complementary,and greatly improves the hepatitis C virus detection rate,providing a powerful basis for clinical early and accurate diagnosis of hepatitis C virus ,condition monitoring,and clinical observation, and providing a strong support for screening blood donors and safety of blood products.

关 键 词：丙型肝炎病毒核酸 丙型肝炎病毒抗体 荧光定量逆转录聚合酶链反应 酶联免疫吸附试验 丙氨酸氨基转移酶 

分 类 号：R446[医药卫生—诊断学] R512.33[医药卫生—临床医学]