高效液相色谱法研究注射液加替沙星在家兔体内的药代动力学  

Pharmacokinetics study of Gatifloxacin injection in vivo rabbit by HPLC

作　　者：刘海燕[1] 张云杰[1] 张楠楠[1] 柴佳丽[1] 

机构地区：[1]佳木斯大学化学与药学院,黑龙江佳木斯154007

出　　处：《黑龙江医药科学》2008年第3期32-34,共3页Heilongjiang Medicine and Pharmacy

摘　　要：目的:建立测定家兔血清中加替沙星药物浓度的高效液相方法,进一步研究注射液加替沙星在家兔体内的药代动力学。方法:通过对健康家兔耳缘静脉注射加替沙星后,分别在1,3,5,7.5,15,30,60,120,180,240,360m in时进行血液采集,用高效液相色谱法中的定量分析。结果:加替沙星C0=39.6947,药物的分布半衰期t1/2a=0.0215,药物的消除半衰期t1/2b=1.4292,K12=23.8976,K21=6.2912,K e l=2.4807,Clb=1.2499,AUC=16.0017,Vd=2.5776,单剂量静脉注射二室模型表达式:C=32.424e(-32.1846t)+7.2707e(-0.4849t)。结论:本法快速、准确,重现性好,为该药物的临床血药浓度监测和药代动力学研究提供依据。Objective：To establish a method used to determine medicine concentration in rabbit serum by HPI.C and further study Gatifloxacin injection pharmacokinetics in vivo rabbit. Method：After injecting Gatifloxacin to ear edge by intravenous injection,we made quantitative analysis by HPLC to collect blood at 1,3,5,7. 5,15,30,60,120,180,240 and 360 min, respectively. Results ：Gatifloxacin Co = 39. 6947 ,medicine distribution half life t1/2a = 0. 0215, elimination half life t1/2b= 1. 4292,K12 = 23. 8976,K21 =6. 2912,Kel= 2. 4807 ,C1b = 1. 2499, AUC= 16. 0017 ,and Vd = 2. 5776. And two--compartment model expression of single dose intravenous injection：C=32.424e（-32.1846t）＋7.2707e（-0.4849t）. Conclusion. It is a rapid and accurate method with better reproducibility. Therefore, the concentration in blood and pharmacokinetics. method can offer a basis for monitoring clinic medicine concentration in blood and pharmacokinetics.

关 键 词：加替沙星 药代动力学 高效液相色谱法 

分 类 号：R969.1[医药卫生—药理学]