用流加培养技术在光生物反应器中培养新月菱形藻的研究  

Fed-batch Culture of alga Nitzschia closterium in a photobioreactor

作　　者：任莉红[1] 孙利芹[1] 王长海[1] 

机构地区：[1]烟台大学海洋学院,山东烟台264005

出　　处：《大连水产学院学报》2008年第3期210-214,共5页Journal of Dalian Fisheries University

基　　金：农业部暨辽宁省省级高校海洋水产增养殖与生物技术重点开放实验室开放课题(K2006-03)

摘　　要：应用微生物流加培养技术对新月菱形藻Nitzschia closterium分别进行恒速流加培养、变速流加培养,并与一次性培养进行了比较。结果表明:不同的流加方式对新月菱形藻的生长、蛋白质、胞内多糖产量影响显著;恒速流加培养10 d的藻密度、蛋白质及多糖的含量分别为(1.901±0.050)×107cell/mL、(42.82±4.03)mg/L、(95.68±3.99)mg/L,是一次性培养的2.29、1.62、2.15倍;在变速流加培养中,当流加控制因子K=0.167时,藻密度、蛋白质、多糖的含量分别是一次性培养的4.83、6.23、6.12倍。The batch and fed -batch culture methods were employed to investigate the effects of culture mode on growth of alga Nitzschia closterium. The results showed that the different fashions of fed - batch culture had distinct effects on growth and production of protein and polysaccharide in the alga. When the alga was fed - cultured with invariable fedvelocity, the cell density, contents of protein and polysaccharide at the 10^th day were （1. 901 ± 0. 043 ） × 10^7 cell/mL, （42.82 ± 4.03 ） mg/L, and （ 95.68 ± 3.99 ） mg/L, respectively, equal to 2.29 times, 1.60 times and 2.15 times of batch culture, respectively. When the feeding rate of fed -batch was variable, the biomass of the alga at the fed - rate of 0. 167 was about 4.83 times higher than that cultured by method of batch culture. Likewise, the contents of protein and polysaccharide increased by 6.23 times and 6.12 times, respectively.

关 键 词：新月菱形藻 流加培养 生物量 蛋白质 多糖 

分 类 号：Q949.27[生物学—植物学]