房颤犬心房组织转化生长因子β_1和血管紧张素转化酶的表达及意义  

Expression of transforming growth factor β_1 and angiotensin-convertion enzyme in dog of atrial fibrillation

作　　者：董蕾[1] 刘元生[2] 李燕[3] 郭继鸿[2] 

机构地区：[1]北京大学人民医院内科 [2]北京大学人民医院急诊科,北京100044 [3]山西医科大学第二医院急诊科,山西太原030001

出　　处：《临床荟萃》2008年第14期1003-1005,F0003,共4页Clinical Focus

基　　金：国家自然科学基金(30271431);北京市自然科学基金(7032030)

摘　　要：目的探讨促心房纤维化因素转化生长因子β1(TGF-β1)和血管紧张素转化酶(ACE)与心房颤动(AF)的相关性。方法健康杂种犬16条,随机分为对照组(n=8)与AF组(n=8)。AF组置入永久起搏器,起搏时间24周。对照组不植入起搏器。制备AF犬模型。在左、右心房切取组织块,甲醛固定后,石蜡包埋保存。两组石蜡切片均行免疫组织化学检测TGF-β1和ACE,比较两组间TGF-β1、ACE的表达水平。结果以平均光密度表示各组TGF-β1、ACE的表达水平,进行定量分析。结果显示:AF组TGF-β1的平均光密度测定值明显高于对照组(19.00±6.44)%vs(11.07±6.27)%(P<0.01);AF组ACE的平均光密度测定值明显高于对照组(8.73±3.86)%vs(5.52±2.66)%(P<0.01)。结论TGF-β1和ACE是促进心房纤维化形成的重要因素,可能与AF的发生和维持密切相关。Objective To investigate whether atrial expression of transforming growth factor β1 （TGF-β1） and angiotensin-convertion enzyme（ACE） is altered in dogs with atrial fibrillation（AF）. Methods Sixteen mongrel dogs were randomly divided into two groups：paclng group（ n=8） and control group（ n=8）. High frequency pacing （500±20） times/min was performed in pacing group with permanent pacemaker. Pacing was not performed in control group. After pacing to 24 weeks,tissue samples were obtained from the left and right atria when dogs were killed. The level of TGF-β1 and ACE in atria were detected by immunohistochemistry. Results Compared to those of control group, the expression levels of TGF-β1 and ACE were significantly enhanced in atrial tissues during AF in pacing group, （19.00±6.44）% vs （11.07±6.27）%,（8.73±3.86）% vs （5.52±2.66）%（P〈0.01）. Conclusion TGF-β1 and ACEin dog participate in AF, which may be one of the main mechanisms in the genesis and development of AF with pomoting atrium fibrosis.

关 键 词：心房颤动 心脏起搏器 人工 转化生长因子Β1 血管紧张素转化酶 免疫组织化学 

分 类 号：R541.75[医药卫生—心血管疾病]