耐甲氧西林金黄色葡萄球菌耐药性分析  

Study on insecticide tolerance of Methicillin resistant Staphylococcus aureus

作　　者：房强[1] 

机构地区：[1]长春工业大学生物工程学院,吉林长春130012

出　　处：《吉林医学》2008年第11期883-884,共2页Jilin Medical Journal

摘　　要：目的:分析耐甲氧西林的金黄色葡萄球菌(MRSA)检测方法及其耐药性。方法:采用头孢西丁纸片法和PCR两种方法检测临床分离的60株金黄色葡萄球菌中MRSA菌株;纸片扩散法测定金黄色葡萄球菌对15种抗菌药物的敏感性。结果:纸片法及PCR法对MRSA检出率分别为15%、13.3%,两种方法对MRSA检出率差异无统计学意义(P<0.05)。以PCR法检出mecA、femB基因为判断MRSA的金标准。除了替考拉宁、万古霉素以外,其余抗生素的耐药率MRSA组均明显高于MSSA组(P<0.01)。结论:头孢西丁纸片法是筛选和确认MRSA菌株的一种可靠、简便的方法;MRSA为多重耐药菌株,临床应加强检测。Objective To analyze differentiate of detecting Methicillin resistant Staphy lococeus aureus （MRSA） and investigate the changes of antimicrobial susceptibility of staphylococcus aureus in order to guide a reasonable use of antibiotics. Methods Sixty strains of S. aureus isolated from clinical specimens were tested for K - B to 15 antibiotics. Results The isolate rate of K - B and PCR method was 15% and 13.3%, respectively, which there was not significant difference （ P 〉 0.05）. The double - PCR was goaden criterion for MRSA. Except for Vancomyem and Teicoplanin , the susceptiblility of MRSA was lower than MSSA. Conclusion K - B for Cefoxitin is a reliable and convenient method. Surveillance of the susceptibility of provincial S. aureus to antimicrobial agents is of great importance to both rational use of antibiotics and reduction of resistant strains.

关 键 词：耐甲氧西林 金黄色葡萄球菌 R4/E3C 

分 类 号：R446.5[医药卫生—诊断学]