大鼠睾丸支持细胞体外培养及FSH对其增殖的影响  

Cultivation of rat sertoli cell in vitro and effect of FSH on its proliferation

作　　者：李玉荣[1,2] 白瑜[1] 尹晓敏[1] 周向梅[1] 韩利方[1] 赵德明[1] 

机构地区：[1]国家动物传染海绵状脑病实验室,中国农业大学动物医学院,北京100094 [2]河北农业大学动物科技学院,河北保定071000

出　　处：《河北农业大学学报》2008年第3期73-76,共4页Journal of Hebei Agricultural University

基　　金：国家自然科学基金(30571399);教育部平台基金(2006BAD06A13)

摘　　要：为探索睾丸支持细胞体外纯化培养的方法,并观察不同水平FSH对支持细胞增殖能力的影响,本研究采用胶原酶Ⅰ和胰蛋白酶序贯两步消化法和低渗处理法分离、纯化睾丸支持细胞,Fas-L免疫荧光法对纯化后的支持细胞进行鉴定。取培养第2代第2天已完全贴壁的支持细胞,在其培养液中添加不同浓度FSH,培养16 h后,MTT法检测FSH对支持细胞增殖能力的影响。结果表明,该方法培养的支持细胞纯度高,且FSH的添加可显著促进支持细胞的增殖(P<0.01),为支持细胞高效制备提供可行性。Rat sertoli cells were isolated and cultured in vitro, and effects of FSH on the proliferation of the cultured sertoli cells were investigated. Tripsin and collagenase were used for harvesting and processing of the sertoli cell, and then the purity of sertoli cells was identified by the immunofluorescence of Fas - L. Following culture of the above - prepared cells for 2 days, sertoli cells were incubated with the presence of the different concentration of FSH, after culture for 16 h , the proliferation of sertoli cells was determined by MTT assay. Results show the culture method is effective for sertoli cells of high purity ; FSH has significantly stimulated sertoli cell proliferation （ P 〈 0.01 ）. The conclusion could contribute to the cultivation of rat sertoll cell in vitro.

关 键 词：大鼠 睾丸 支持细胞 培养 卵泡刺激素(FSH) 增殖 

分 类 号：Q813.1[生物学—生物工程]