高活性微生物絮凝剂产生菌的筛选与培养条件优化  

Screening and Culture Condition Optimizing of Microbe with High Flocculating Capability

作　　者：赵继红[1] 刘从彬[1] 李佳[1] 

机构地区：[1]河南工业大学化学化工学院,郑州450001

出　　处：《河南大学学报（自然科学版）》2008年第4期393-396,405,共5页Journal of Henan University:Natural Science

基　　金：郑州市科技攻关计划项目资金资助(052SGYS31193)

摘　　要：从郑州市五龙口污水处理厂活性污泥中分离出8株具有絮凝活性的微生物菌株,复筛得到1株高活性微生物絮凝剂产生菌,测定了该菌株不同培养条件下发酵液对高岭土悬浊液的絮凝率.结果显示,蔗糖20 g/L,NaNO31.5 g/L,K2HPO41 g/L,KCl 0.5 g/L,MgSO4.7H2O 0.5 g/L,FeSO40.01 g/L,pH 12,温度35℃,摇床转速150 r/min,培养时间74 h为该菌株的最适培养条件.在该培养条件下,该菌株对高岭土悬浊液的絮凝率达94.98%.Eight microbial flocculant-producing bacteria were screened from active sludge of Zhengzhou Wulongkou wastewater treatment plant. One of them was proved to have high flocculating capability through re-screened and was named as the isolate 127^# . The optimal culture medium and the culture conditions of the isolate 127^# were established as follows： sucrose 20 g/L, sodium nitrate 1.5 g/L, dipotassium hydrogen phosphate 1 g/L, potassium chloride 0.5 g/L, heptahydrate magnesium sulfate 0.5 g/L, ferrous sulfate 0.01 g/L; pH 12, temperature 35 ℃, shaker rotate speed 150 r/rain, culture time 74 h. Under the optimal culture conditions, the flocculating ratio was up to 94.98%.

关 键 词：微生物絮凝剂 筛选 培养条件 

分 类 号：Q935[生物学—微生物学]