软肝冲剂对肝纤维化大鼠组织金属蛋白酶抑制剂的影响  

Resolution of CCl_4-induced rat liver fibrosis via TIMP-1 expression inhibition by Ruangan granule

作　　者：莫其农[1] 黄涛[1] 方晓艺[2] 周小梅[1] 何进才[1] 贺劲松[3] 周小舟[3] 童光东[3] 熊益群[3] 周大桥[3] 

机构地区：[1]广州中医药大学附属深圳市中医院检验科,广东深圳518033 [2]广州中医药大学附属深圳市中医院体检中心,广东深圳518033 [3]广州中医药大学附属深圳市中医院肝病研究所,广东深圳518033

出　　处：《中西医结合肝病杂志》2008年第3期160-163,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：深圳市科技局立项项目(No.JH200507130998A)

摘　　要：目的:探讨软肝冲剂治疗肝纤维化和肝硬化的机制是否与抑制TIMP-1的表达有关。方法:用CCl4诱导大鼠的肝纤维化模型(病理分期为0～Ⅴ期),将大鼠随机分成两组,分别用软肝冲剂和生理盐水治疗2个月后,对肝组织TIMP-1mRNA的表达进行分析,并检测羟脯氨酸(Hyp)的量,同时检测血清中TIMP-1和TGF-β1的浓度。结果:软肝冲剂能够降低大鼠肝组织中TIMP-1mRNA的表达和胶原蛋白的含量,同时血清中TIMP-1和TGF-β1较CCl4诱导的模型组和生理盐水治疗组显著降低(P<0·05)。结论:软肝冲剂治疗肝纤维化可能通过阻止TIMP-1的表达起作用。Objective： To study the relation between the effect of Ruangan granule to liver fibrosis and tissue inhibitor of metalloproteinases-1 （TIMP-1） mRNA expression. Methods： CCl4-induced rats were randomly divided into two groups which were treated by Ruangan granule and Normal Saline （NS） respectively. The expression of TIMP-1 mRNA and hydroxyproline in liver tissue was analyzed. And serum levels of TIMP-1 mRNA and TGF-β1 were detected. Results： The expression of mRNA and collagen in liver tissue of CCl4-induced rats treated by Ruanganchengji were significantly decreased, the serum levels of TIMP-1 and TGF-β1 were significantly decreased compared with those of model and NS-treated groups. Conclusion： The mechanism of Ruangan granule treatment for liver fibrosis is possible to be blockade of TIMP-1 expression in liver.

关 键 词：软肝冲剂/药理作用 组织金属蛋白酶抑制剂 肝纤维化 动物模型 

分 类 号：R285.5[医药卫生—中药学]