基因B型及C型慢性乙型肝炎患者特异性T淋巴细胞应答的差异  

Difference of HBV specific T cell responses between chronic HBV infected patients with genotype B and C

作　　者：马世武[1] 彭劼[1] 胡小雄[1] 杨玲[1] 陈金军[1] 于乐成[1] 侯金林[1] 

机构地区：[1]南方医科大学南方医院感染内科,广州510515

出　　处：《广东医学》2008年第7期1082-1084,共3页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:30571162;30471549)

摘　　要：目的比较HBV基因B,C基因型患者间HBV特异性T淋巴细胞应答的差异。方法以我国主要流行的B,C基因型为病毒学背景,设计覆盖HBV全基因序列的重叠多肽池,PCR-RFLP方法检测患者HBV基因型,通过酶联免疫斑点试验检测30例基因B,C型慢性HBV感染者的特异性T淋巴细胞分泌IFN-γ差异。结果在30例慢性HBV感染者中基因B型有21例(70%),基因C型有9例(30%);在特异性T淋巴细胞应答方面,HBV基因B型患者明显高于基因C型患者(P=0.021)。结论HBV基因B型较高的特异性T淋巴细胞反应水平对于解释HBV基因B型患者与基因C型患者临床转归差异提供了细胞免疫学依据。Objective To analyze the difference on HBV specific T cell response between patients with HBV genotype B and C infection. Methods Based on the predominant HBV virological background, overlapping peptide spanned whole HBV genome was synthesized with 10 mers amino acids overlapping, PCR - RFLP approach was performed to determine HBV genotype, and IFN - γ ELISPOT assay was set up to detect HBV specific T cell response in 30 inpatients with HBV genotype B or C infection. Results There were 21 patients with genotype B infection and 9 with genotype C infection; compared with patients with genotype C infection, the patients with genotype B infection showed higher specific T cell response（P = 0. 021 ）. Conclusion The higher specific T cell response in genotype B infected patients maybe reveal a cellular immunological evidence on outcome difference between patients with genotype B infection and genotype C infection.

关 键 词：乙型病毒性肝炎 基因型 细胞免疫应答 重叠多肽 

分 类 号：R512.8[医药卫生—内科学] R512.62[医药卫生—临床医学]