热致相分离法制备食道组织工程用支架的研究  

Fabrication of Asymmetrical Porous Scaffold in Tissue Engineering Using Thermally Induced Phase Separation Method

作　　者：竺亚斌[1] 刘玉新[1] 

机构地区：[1]宁波大学医学院,浙江宁波315211

出　　处：《宁波大学学报（理工版）》2008年第2期240-245,共6页Journal of Ningbo University：Natural Science and Engineering Edition

基　　金：宁波市自然科学基金(2006A610053);宁波市社发项目(2007C10015)

摘　　要：合适的支架是进行组织工程研究必需的条件之一.运用自制的具有较好弹性的无规聚合物聚(L-乳酸-co-己内酯,PLLC),并采用热致相分离法(TIPS)制备了具有不对称孔径的食道组织工程用三维支架.经过对不同成孔条件(包括粗化温度、粗化时间以及聚合物溶液浓度等)的比较和研究,使此支架上表面具有孔径10μm,而本体具有50～100μm,甚至100μm的不对称多孔支架.并模拟正常食道粘膜层构造,将提取自猪食道的原代上皮细胞和成纤维细胞分别种植于此支架的二面,经HE常规染色,发现在体外培养时间(14d)内,在支架上表面已形成一连续层的上皮细胞层,某些部位甚至多于一层,这与正常食道的上皮细胞层相似.对于成纤维细胞而言,在与上皮细胞层同一视野内所见成纤维细胞较少,因为如果细胞生长在支架内部就很难用常规方法观察得到.但从这些结果可以看到用PLLC制备的多孔支架对细胞没有毒性,且具有一定的支持细胞生长的作用.Asymmetrical porous 3-D scaffold for esophageal tissue engineering has been fabricated using thermal-induced phase separation method. The scaffold in which there are pores with 1-10 μm diameter on one surface and pores with 50-100μm diameter on the other is used to simulate the mucosa composition of normal esophagus. The porcine primary esophageal epithelial cells are seeded on the surface with smaller pores, and primary fibroblasts are cultured in the bulk. After being in vitro culture for 14 days, one confluent layer of epithelial cell is formed besides multilayer formed somewhere, while fibroblasts are also found in the scaffold bulk. This fact indicates that the present scaflbld can support well the growth of primary epithelial cell and fibroblast. However, there are still some problems with these two types of cells to be cocultured in the present culture system, for example, few fibroblasts are found below the epithelial cell layer, resulting in little interaction between these two types of cells. It might be related to the seeding density, coculture conditions and scaffold properties, etc. Our next work is to further study the fabrication of optimized scaffold for esophageal tissue engineering.

关 键 词：生物高分子材料 不对称孔径支架 热致相分离法 食道组织工程 原代上皮细胞 

分 类 号：TB383[一般工业技术—材料科学与工程]