杜氏藻(Dunaliella sp．)多糖DPS-1的提取分离纯化和糖基组成分析  

Extractation separation purification and component analysis of glycon of DPS-1 from dunaliell

作　　者：李亚清[1] 张华微[2] 杨海波[3] 王心满[2] 崔丽华[2] 石玉红[2] 

机构地区：[1]大连理工大学精细化工国家重点实验室,辽宁大连116012 [2]辽宁省朝阳师范高等专科学校生物化学工程系,辽宁朝阳122000 [3]大连大学化学与化学工程学院,辽宁大连116622

出　　处：《海洋环境科学》2008年第3期217-219,共3页Marine Environmental Science

基　　金：辽宁省博士科研启动基金资助项目(001059)

摘　　要：以实验室培养的新鲜的杜氏藻为原料,采用冻结-融化冷水提取、乙醇沉淀,Sephadex G-100柱层析等一系列步骤,分离纯化得到杜氏藻多糖,简称DPS-1。醋酸纤维素薄膜电泳和Sephadex G-50柱层析对DPS-1进行纯度鉴定,结果表明该糖组分均一。IR、UV等理化性质测定及HPAEC-PAD分析,结果表明该糖含有硫酸根,糖环为吡喃环。组成DPS-1的糖基有:D-岩藻糖、D-鼠李糖、2-D-氨基葡萄糖、D-半乳糖、D-葡萄糖和两种未知单糖。其摩尔比为D-岩藻糖∶D-鼠李糖∶2-D-氨基葡萄糖∶D-半乳糖∶D-葡萄糖=2.1∶1∶1.6∶2.3∶4.2。另外还检出了两种已知糖醛酸,分别是半乳糖醛酸和葡萄糖醛酸。其摩尔比为半乳糖醛酸∶葡萄糖醛酸=1∶3.4。The Dunaliell polysaccharide was extracted through a series of steps ： taking cultured fresh Dunaliell as raw material, crash- ing the cells by frozen-thawn, extracting it with cold water, ethanol precipitation and Sephadex G-100 column chromatography. And it is called DPS-1 for short. The cellulose acetate membrane electrophoresis and the Sephadex G-50 purity test of it show that the polysaccharide is very uniform. The physical chemistry test of IR and UV and the HPAEC-PAD glycon component analysis show that the polysaccharide is a kind of acetamine , and its rings of sugar may be pyran rings. The determined glycons of the polysaccharide are Dfucose, D-rhamnose, D-2-glucosamine, D-galactose and D-glucose and their mole ratio are 2.1 ： 1.0： 1.6： 2.3：4.2 correspondingly. There are also two determined uronic acids： galacturonic acid and glucuronic acid and their mole ration are 1：3.4.

关 键 词：杜氏藻 多糖 糖基 组成 

分 类 号：Q53[生物学—生物化学] Q949