室旁核注射apelin-13对大鼠胃缺血/再灌注损伤的影响  

Effects of apelin-13 microinjection into the hypothalamic paraventricular nucleus on gastric ischemia/reperfusion injury in rats

作　　者：丁雷[1] 张建福[2] 张咏梅[2] 

机构地区：[1]徐州医学院病理生理学教研室,江苏徐州221002 [2]徐州医学院生理学教研室

出　　处：《徐州医学院学报》2008年第6期357-359,共3页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金(30570671);江苏省教育厅基金(05KJB310134)

摘　　要：目的研究室旁核注射apelin-13对胃缺血/再灌注(GI/R)损伤的作用及细胞机制。方法室旁核注射apelin-13,制备GI/R模型;用Western-blot方法检测胃黏膜细胞的凋亡以及凋亡相关基因c-Jun氨基末端激酶(c-Jun N-terminal kinase,JNK)的磷酸化水平。结果与单纯GI/R组相比,室旁核注射apelin-13能明显增加GI/R后胃黏膜细胞的损伤,同时可以升高caspase-3蛋白表达及JNK的磷酸化水平。结论室旁核注射apelin-13对大鼠GI/R损伤具有加重作用。Objective To observe the effects and mechanism of apelin - 13 microinjection into the hypothalamic paraventricular nucleus （PVN） on gastric ischemia/reperfusion （GI/R） injury in rats. Methods Apelin - 13 was first microinjected into the PVN before the establishment of GI/R model, which was carried out in rats by clamping the celiac artery for 30 min and the following reperfusion for 1 h. Western - blot was used to determine the expressions of caspase - 3 and p - JNK of the gastric mucosal cells. Results Compared with the GI/R control group, apelin - 13 microinjection into the PVN markedly aggravated the damage in gastric mucosa and enhanced the expressions of caspase - 3 and p-JNK. Conclusion Apelin - 13 microinjection into PVN can aggravate the harmful effect on gastric mucosa induced by GI/R.

关 键 词：APELIN-13 室旁核 胃缺血/再灌注 细胞凋亡 C-JUN氨基末端激酶 

分 类 号：R426[医药卫生—临床医学] R338.27