甲泼尼龙对肺炎支原体诱导A549细胞分泌IL-8和TNF-α的影响  

Effect of Methylprednisolone on Cytokine IL-8 and TNF-α Secretions in A549 Cells Induced by Mycoplasma pneumonia

作　　者：吴小兰[1] 刘先洲[1] 汤纪路[1] 朱芮[2] 杨杨[3] 

机构地区：[1]武汉大学基础医学院微生物学教研室 [2]武汉大学中南医院儿科,武汉430071 [3]武汉科技大学临床学院,武汉430064

出　　处：《华中科技大学学报（医学版）》2008年第3期295-298,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的观察甲泼尼龙(MPL)对肺炎支原体(MP)诱导A549细胞产生细胞因子白细胞介素-8(IL-8)和肿瘤坏死因子-α(TNF-α)的影响,为临床在MP肺炎中合理应用糖皮质激素(GCs)提供理论依据。方法体外培养A549细胞和MP,用不同感染复数的MP诱导A549细胞,将0.015%的MPL加入到MP诱导的A549细胞中共同孵育,采用双抗体夹心ELISA法检测MPL作用于A549细胞后细胞分泌的IL-8和TNF-α的含量。结果MP能显著诱导A549细胞分泌IL-8和TNF-α,0.015%MPL能明显抑制A549细胞分泌IL-8和TNF-α(P<0.01),随着MP感染复数的增加,MPL的抑制作用降低。结论GCs可抑制MP诱导的A549细胞IL-8和TNF-α的分泌,提示在重症MP肺炎早期,适当应用合理剂量的GCs可减轻炎症反应的强度。Objective To observe the effect of methylprednisolone （MPL） on cytokine IL-8 and TNF-α secretion in A549 cells induced by Mycoplasma Pneumonia （MP）. Methods A549 cells and MP were co-cultured in vitro. 0. 015% MPL was added into A549 cells which were pre-induced with MP at different multiplicity of infection （MOI）. Enzyme-linked immunosorbent assay （ELISA） was used to detect the contents of IL-8 and TNF-α before and after MPL treatment, Results MP could significantly induce IL-8 and TNF-α secretion in A549 cells, The contents of IL-8 and TNF-α were greatly depressed after 0. 015% MPL treatment （P〈0.01）, and the inhibitory effect of MPL was attenuated with the increase of MOI of MP. Conclusion Glucocorticoids can obviously inhibit the MP-induced A549 cells to secrete IL-8 and TNF-α, suggesting that the clinical application of gluococorticoids can attenuate the inflammatory response of severe MP in early stage.

关 键 词：肺炎支原体 甲泼尼龙 白细胞介素-8 肿瘤坏死因子-α 

分 类 号：R563.13[医药卫生—呼吸系统]