肿瘤坏死因子α对Raji细胞Notch1蛋白表达的影响  

Expression of TNF-α on Notch1 Expression in Raji Cells

作　　者：崔国惠[1] 陈卫华[1] 陈燕[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院血液科,武汉430022

出　　处：《华中科技大学学报（医学版）》2008年第3期299-301,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No30472267)

摘　　要：目的通过探讨肿瘤坏死因子α(TNF-α)作用淋巴瘤细胞系Raji细胞后Notch1蛋白表达的变化,进一步明确TNF-α对淋巴瘤细胞Notch信号途径的影响。方法采用半定量RT-PCR方法检测Raji细胞Notch1 mRNA的含量;用流式细胞术分析Raji细胞Notch1蛋白的表达及TNF-α作用后Notch1蛋白的变化。结果①半定量RT-PCR检测结果表明Raji细胞经TNF-α作用后Notch1mRNA的含量明显增高,且随TNF-α浓度的增加而增高,与对照组比较,差异有极显著性意义(P<0.01);②流式细胞术分析表明,Notch1蛋白在Raji细胞呈阳性表达,TNF-α作用组Notch1蛋白的平均荧光强度明显高于对照组,差异亦有极显著性意义(P<0.01)。结论TNF-α可上调Raji细胞内Notch1蛋白的表达;TNF-α可通过影响Notch信号途径在淋巴瘤细胞内发挥作用。Objective To investigate the effect of TNF-α on the expression of Notch1 in Raji cell line. Methods The expression level of Notch1 mRNA and protein in Raji cells treated with TNF-α was detected by RT-PCR and flow cytometry respectively. Results The expression of Notch1 mRNA was up-regulated in a dose-dependent manner in Raji cells treated by TNF-α （P〈0.01）. Raji cells treated with TNF-α had a higher fluorescence intensity of Notch1 protein as compared with untreated cells （P〈0.01）. Conclusion Notch1 protein was expressed in Raji cells and the expression of Notch1 protein was increased after TNF-α treatment, suggesting that activated Notch1 signaling pathway plays an important role in pathogenesis of lymphoma and it might be a potential new target for treatment.

关 键 词：肿瘤坏死因子Α NOTCH1蛋白 RAJI细胞 

分 类 号：R979[医药卫生—药品]