EphB/Ephrin-B1信号对睫状神经节α3-nAChRs电流的影响  

Effects of EphB/Ephrin-B1 Signal on the Currents of α3-nAChRs in Ciliary Ganglion Neurons

作　　者：胡波[1] 童萼塘[1] 梅元武[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院神经内科,武汉430022

出　　处：《华中科技大学学报（医学版）》2008年第3期324-328,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No30600189)

摘　　要：目的探讨EphB/Ephrin-B1信号对鸡胚睫状神经节(ciliary ganglion,CG)神经元上α3-尼古丁乙酰胆碱受体(nicotinic acetylcholine receptors,α3-nAChRs)电流的影响。方法用膜片钳技术分别在培养的和急性分离的CG神经元上记录α3-nAChRs电流,细胞被随机分为:对照组;Ephrin-B1Fc治疗组——用Ephrin-B1与IgG重组后形成的Ephrin-B1Fc刺激细胞;IgG治疗组——用重组Ephrin-B1Fc所需相同浓度的IgG刺激细胞;Ephrin-B1治疗组——用重组Ephrin-B1Fc所需相同浓度的Ephrin-B1刺激细胞,观察电流的变化。结果对照组、IgG治疗组和Ephrin-B1治疗组之间α3-nAChRs电流无显著差异(P>0.05),而此3组与Ephrin-B1Fc治疗组之间有显著差异(均P<0.05),Ephrin-B1Fc可浓度依赖性抑制α3-nAChRs电流,该过程可快速发生并呈现可逆性。培养的和急性分离的CG神经元Ipeak/Cm的IC50分别为4.9μg/ml和8.4μg/ml。结论本研究表明Eph/Ephrin-B1信号可影响CG神经元α3-nAChRs的电流,提示Eph/Ephrin-B1可能参与交感神经系统功能的调控。Objective To investigate the effect of EphB/Ephrin-B1 signal on the currents of α3-nicotinic acetylcholine receptors （α3-nAChRs） on ciliary ganglion neurons. Methods Currents of α3-nAChRs on cultured and acute-associated ciliary ganglion neurons were recorded by using patch clamp technique. Cells were randomly divided into the following groups： control group, Ephrin-B1Fc-treated group （stimulating cells with different concentrations of recombinant Ephrin-B1Fc）, IgG-treated group （stimulating cells with IgG）, Ephrin-B1-treated group （stimulating cells with Ephrin-B1）. The changes in currents were observed. Results There was no significant difference in the currents of α3-nAChRs between control, IgG-treated and Ephrin- B1-treated groups, but there was significant difference between these three group and Ephrin-B1Fc-treated group （all P〈 0. 05）. Ephrin-B1Fc could concentration-dependently inhibit the currents of α3-nAChRs and the inhibitory effects happened quickly and were reversible. In the cultured and acute-associated ciliary ganglion neurons, IC50 of Ipeak/Cm was 4.9 and 8.4ug/ ml, respectively. Conclusion EphB/Ephrin-B1 signal can affect currents of α3-nAChRs on ciliary ganglion neurons, suggesting that EphB/Ephrin-B1 signal may play some important roles in regulation of sympathetic nervous system.

关 键 词：α3-nAChRs电流 EphB/Ephrin-B1信号 睫状神经节神经元 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]