久效磷人工抗原的制备、鉴定与免疫效价的测定  

Preparation and Identification of Artificial Antigen for Monocrotophos and Determination of Immune Titers

作　　者：夏莹[1] 李中振[1] 杨迪[1] 杨旭[1] 丁书茂[1] 袁均林[1] 

机构地区：[1]华中师范大学生命科学学院环境科学实验室,湖北武汉430079

出　　处：《化学与生物工程》2008年第6期38-40,45,共4页Chemistry & Bioengineering

基　　金：国家科技部建设部科技支撑计划资助项目(2006BAJ02A10);国家科技部卫生部科技支撑计划资助项目(2006BAI19B05)

摘　　要：以戊二醛为交联剂制备了久效磷(Mcp)人工抗原(Mcp-OVA)和包被抗原(Mcp-BSA),采用紫外吸收光谱和SDS-PAGE对人工抗原进行了鉴定。取人工抗原免疫BALB/c小鼠,并采用ELISA法对小鼠抗血清效价进行了检测。结果表明,人工抗原中Mcp与OVA的偶联比为23.3∶1,Mcp-OVA的最适包被浓度为1.5μg.mL-1,3次免疫后小鼠的抗血清效价均达到1∶2×105以上。Monocrotophos （Mcp） was conjugated to BSA and OVA via glutaraldehyde to make artificial antigen and coating antigen,which were identified by UV spectrometry and SDS-PAGE. BALB/c mice immunized by artificial antigen were identified by ELISA. The results showed that the conjugated ratio of Mcp to OVA in Mcp-OVA was 23.3 ： 1, the optimum coating concentration for Mcp-OVA was 1.5 μg · mL^-1. And the titers of antiserum reached above 1 ： 2 × 10^5 when the mice were immunized for three times.

关 键 词：久效磷 人工抗原 抗血清 

分 类 号：TQ450.2[化学工程—农药化工] Q503[生物学—生物化学]