毛柄金钱菌gpd-Fv启动子的克隆及序列分析  

Cloning and sequence analysis of gpd-Fv promoter from F. velutipes

作　　者：杨培周[1] 郭丽琼[1] 王艺红[1] 林俊芳[1] 

机构地区：[1]华南农业大学食品学院生物工程系

出　　处：《工业微生物》2008年第3期33-37,共5页Industrial Microbiology

基　　金：国家自然科学基金(30671457;30371000);国家高技术研究发展计划(863计划;2006AA10Z301)

摘　　要：根据Genbank登陆的毛柄金钱菌gpd启动子序列设计引物,对毛柄金钱菌基因组DNA进行PCR扩增,扩增获得了2条特异片段gpd-Fv1和gpd-Fv2;回收特异片段后分别克隆进T-easy载体中并进行DNA序列测序,结果表明:gpd-Fv1和gpd-Fv2长度分别为1412 bp和752bp。通过同源性分析,结果表明:gpd-Fv1和gpd-Fv2的DNA序列与已报道的毛柄金钱菌gpd启动子(Genbank:AF515622.1)的同源性分别为95%和98%。进一步通过软件预测,结果表明克隆的2条片段具有基本的启动子结构,存在多个转录因子结合位点。根据启动子的序列特点,初步构建了毛柄金钱菌gpd启动子模型。Two fragments were amplified by PCR with two pairs of primers based on the reported gpd promoter sequence （accession number： AF515622.1） in Genbank from the genomic DNA of Flammulina velutipes. DNA sequencing result indicated that the fragments, gpd-Fvl and gpd-Fv2 were 1412bp and 752bp in length, respectively. Blast search result showed that the gpd-Fv1 and gpd-Fv2 had 95% and 98% similarities respectively with the reported F. velutipes gpd promoter sequence in Genbank. The promoter predictive software analysis showed that two promoters possess basic structural characteristics of promoter with a few of binding sites of transcriptional factors. The F. velutipes gpd-Fv promoter model was also constructed based on its sequence characteristics.

关 键 词：毛柄金钱菌 启动子 克隆 序列分析 模型 

分 类 号：Q78[生物学—分子生物学] S646.19[农业科学—蔬菜学]