高酯酶活性工程菌的构建及其对有机磷农药的降解  

Construction of engineered bacteria with high enzyme activity and degradation of phosphorus pesticide

作　　者：张丽青[1] 闫艳春[2] 姜红霞[3] 张庆乐[1] 唐心强[1] 

机构地区：[1]泰山医学院化学与化学工程学院,山东泰安271016 [2]中国农业科学院研究生院,北京100081 [3]泰山医学院药学院,山东泰安271016

出　　处：《工业微生物》2008年第3期47-50,55,共5页Industrial Microbiology

摘　　要：将抗性库蚊解毒酶酯酶B1基因片段引入融合表达载体pThioHisA中,转化入大肠杆菌DH5α,在IPTG诱导下,经过8 h,酯酶B1在大肠杆菌中获得融合高效表达,目的蛋白占菌体总蛋白的48.7%。重组质粒pThioHisA-B1表达的酯酶融合蛋白具有较高的酯酶B1活性,能高效降解酯酶的特异性底物β-乙酸萘酯(-βNA),并在37℃、pH7.5的条件下,2h内对1000mg/L的甲基对硫磷降解率达81%。The detoxifying gene esterase B1 was cloned into fusion expression vector pThioHisA. The recombinant vector pThioHisA-B1 was constructed and transformed into E. coli DH5α. High level expression of the target protein was obtained in 8 hours after induced by IPTG. The protein expressed by pThioHisA-B1 possessed high esterase activity and it could degrade the specific substrate β-NA of esterase effectively. The results showed that the degradative rate for methylparathion at 1000mg/L by the engineered bacteria was 81% within 2 hours.

关 键 词：酯酶基因 融合表达 工程菌 生物降解 

分 类 号：X592[环境科学与工程—环境工程]