富勒烯[60]膦酸酯衍生物纳米颗粒水悬液的制备与自由基清除活性  

Preparation and Radical Scavenging Activity of an Aqueous Nanoparticle Suspension of a Phosphonate [C_(60)]fullerene

作　　者：杨新林[1] 王自强[1] 黄成[1] 姚璐[1] 宋高广[1] 

机构地区：[1]f北京理工大学生命科学与技术学院,北京100081

出　　处：《有机化学》2008年第6期1061-1064,共4页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(No20672012)资助项目

摘　　要：自由基清除是富勒烯及其衍生物的一种重要特性.首次制备了1种二加成亚甲基富勒烯[60]膦酸酯衍生物(bis-methanophosphonate [60]fullerene,BMPF)的纳米颗粒水悬液(n-BMPF),并采用邻苯三酚自氧化法结合分光光度法,测定了n-BMPF对超氧阴离子自由基的清除作用.结果显示,制备的n-BMPF溶液较稳定,适合生物学实验.同时,它表现出很强的清除超氧阴离子自由基的能力,并具有浓度依赖性.当n-BMPF的终浓度为12μmol/L时,其对超氧阴离子自由基的清除率达到91.96%.n-BMPF的活性远大于已报道具有自由基清除作用的水溶性富勒烯衍生物富勒醇.这些数据表明n-BMPF可能作为一种新型高效的自由基清除剂,在生物医学领域具有潜在的应用价值.Free radical scavenging is an important property of fullerenes and their derivatives. In this paper, an aqueous nanoparticle suspension of a bis-methanophosphonate fullerene （n-BMPF） was prepared for the first time. By using a pyrogallol auto-oxidation method combined with spectrophotometry, the activity of n-BMPF against a superoxide anion radical was determined, finding that n-BMPF obtained was considerably stable and suitable for biological assay. In addition, n-BMPF has an excellent efficiency in eliminating superoxide radicals and the effect is dose-dependent. At a concentration of 12 μmol/L, the scavenging efficiency of n-BMPF is 91.96%. Furthermore, n-BMPF has a much higher activity than fullerenol, a water soluble fullerene derivative which has been reported to be able to eliminate free radicals. These data revealed a potential use of BMPF as the novel potent free radical scavengers in biomedical filed.

关 键 词：二加成亚甲基富勒烯60二膦酸四乙酯 纳米颗粒水悬液 超氧阴离子自由基 

分 类 号：R94[医药卫生—药剂学]