^12C^6＋照射对小鼠睾丸组织脂质过氧化、SOD活性及细胞周期的影响  

Effects of ^(12)C^(6+) irradiation of mouse abdomen on lipid peroxidation,SOD activities and cell cycle of testis

作　　者：龙静[1] 张红[1] 刘阳[1] 赵卫平[1] 谢漪[1] 武振华[1] 

机构地区：[1]中国科学院近代物理研究所

出　　处：《核技术》2008年第6期445-448,共4页Nuclear Techniques

基　　金：国家自然科学基金面上项目(10675151);甘肃省科技攻关计划项目(2GS052-A43-008-2);甘肃省重大科技专项项目(2GS063-A43-012);兰州市-中国科学院高新技术“种子资金”项目(06-2-58)资助

摘　　要：为评估重离子照射的生殖毒性,探讨其损伤的可能机制,用0、0.5、1、2或3Gy剂量12C6+离子对性成熟昆明雄鼠下腹部进行局部照射,6h后处死小鼠,取睾丸组织,用流式细胞术检测睾丸细胞凋亡率和细胞周期,用化学试剂盒检测组织中超氧化物岐化酶(SOD)的活性以及脂质过氧化产物丙二醛(MDA)水平。结果表明,与对照组(0Gy)相比,低剂量照射(0.5Gy)组中睾丸组织SOD活性增加(P>0.05),但SOD活力随剂量增加而下降,在2Gy和3Gy照射组中呈显著抑制作用(P<0.05);睾丸组织中MDA含量随剂量增加,2Gy和3Gy照射组呈显著性差异(P<0.05)。流式细胞术检测结果显示,1～3Gy12C6+照射可导致睾丸组织中单倍体、二倍体及四倍体细胞数量显著减少(p<0.01),凋亡率明显增加(p<0.01)以及G2/M期阻滞(p<0.01)。提示12C6+照射可能通过自由基损伤途径,诱发细胞凋亡和细胞周期异常,从而造成睾丸组织损伤,生殖能力下降。本实验结果为抗氧化剂和自由基清除剂用于临床重离子治疗中对性腺的防护提供了理论依据。To estimate reproductive risks of heavy ion irradiation and the possible mechanism, Kunming strain mice abdomen were exposed to 0, 0,5, 1, 2 or 3 Gy of ^12C^6＋ ions. The DNA content, percentage of each cell-cycle phase and the apoptosis rate were obtained with flow cytometry method. Lipid peroxidation indices, such as malondialdehyde （MDA） level and antioxidants, represented with superoxide dismutase （SOD） activity in the testes were determined by chemical reagent kids. The results showed that SOD activity enhanced slightly in the 0.5 Gy irradiation group （P〉0.05）, but decreased with increasing doses, and significant fall was observed in 2 and 3 Gy on groups comparing with the control （P〈0.05）. The MDA levels increased with the dose, and significant difference was found in the 2 and 3 Gy groups （P〈0.05）. The flow cytometry data showed that 1-3 Gy of ^12C^6＋ ion irradiation resulted in significant decrease in haploid, diploid and tetraploid cells （p〈0.01） and evidently increased apoptosis rate （p〈0.01） in testes tissue. The G2/M phase arrest in irradiation groups were extremely obvious （p〈0.01）. The data suggest that ^12C^6＋ ion irradiation can lead to testes cell high apoptosis rate and cell cycle alteration, which is possibly mediated through oxidative damage induced by over free radicals. The study may provide theoretical basis for application of antioxidants or radical scavengers on protecting gonads from damages during clinical radiotherapy.

关 键 词：^12C^6+辐射 睾丸 超氧化物岐化酶(SOD) 丙二醛(MDA) 流式细胞术 小鼠 

分 类 号：Q691[生物学—生物物理学] Q255