^12C^6＋重离子辐照油菜诱变效应研究  

Mutagenic effects of Brassica napus by ^(12)C^(6+) ion beam

作　　者：侯岁稳[1,2] 孙兰弟[2] 张颖聪[2] 吴大利[2] 管利萍[2] 李文建[1] 党秉荣[1] 颉红梅[1] 周利斌[1] 高清祥[2] 

机构地区：[1]中国科学院近代物理研究所,兰州730000 [2]兰州大学生命科学学院,干旱与草地生态教育部重点实验室,兰州730000

出　　处：《核技术》2008年第6期449-454,共6页Nuclear Techniques

基　　金：中国博士后科学基金(20060390715);“西部之光”联合学者项目(O506160XL0);教育部“新世纪优秀人才支持计划(NCET)”资助

摘　　要：用30Gy、90Gy和180Gy 12C6+重离子辐照油菜干种子,研究其对油菜M1代的诱变效应。结果表明不同剂量12C6+重离子辐照使油菜的出苗率、株高和开花率有不同程度的提高,并使开花期提早;30Gy辐照使单株角果数和单株产量有了一定程度的提高;三种辐照剂量都造成了花粉生活力、千粒重和含油量的降低。RAPD扩增结果表明,42个随机引物中有13个引物扩增出差异条带,30Gy、90Gy和180Gy引起的RAPD变异率分别为22.1%、23.7%和36.2%。研究表明,12C6+重离子辐射能有效地引起油菜DNA序列发生改变,从而诱导基因变异,为油菜育种提供丰富的种质材料。Dry seeds of Brassica napus were irradiated by ^12C^6＋ ion beams with 30, 90, and 180 Gy, and the mutagenic effects of M1 generation were investigated. In comparison to the control group, the irradiated seeds showed positive changes in emerged seedling rate, plant height and blooming rate, with obvious inhibitive effects on pollen vitality, 1000-seed weight and seed oil content. The 30 Gy irradiation group outperformed the others in pods per plant and seed yield per plant. By means of RAPD method, the results showed specific bands, such as increasing bands, flawing bands and bands with different fluorescence intensity were observed in 13 primers selected. The variation rates under 30 Gy, 90 Gy and 180 Gy ^12C^6＋ irradiation were 22.1%, 23.7% and 36.2%, respectively. The research is valuable to the application of ^12C^6＋ heavy ion beam in improving Brassica napus breeding.

关 键 词：^12C^6+重离子 油菜 生物学效应 RAPD 

分 类 号：Q691[生物学—生物物理学]