穿膜肽修饰胸腺五肽类似物的合成和生物活性研究  

Study on the synthesis and biological activities of cell-penetrating peptide modified thymopentin analogues

作　　者：谢文林[1] 杨大成[2] 范莉[2] 彭文烈[1] 徐安龙[1] 

机构地区：[1]中山大学生命科学学院 [2]西南大学化学化工学院,重庆400715

出　　处：《华中师范大学学报（自然科学版）》2008年第2期242-246,共5页Journal of Central China Normal University：Natural Sciences

基　　金：国家高技术研究发展计划(863计划)资助项目(2006AA090504);湖南省教育厅资助科研项目(07B021);湖南科技大学博士启动基金资助项目(E50632)

摘　　要：采用Fmoc固相合成法,利用穿膜肽Tat对胸腺五肽(TP-5)进行修饰,合成了新的胸腺五肽类似物,总收率为32.2%.通过RP-HPLC和质谱对该化合物进行了鉴定,采用E-玫瑰花结实验进行生物活性分析,结果表明:经穿膜肽修饰的类似物在其摩尔浓度稀释20倍后仍然比标准品TP-5具有更强的生物活性.Using the cell-penetrating peptide to modify the thymopentin at N-terminal, the novel thymopentin （TP-5） analogues have been synthesized by a Fmoe solid-phase synthesis strategy. The total yield was 32.2%. The analogues were confirmed by reversed-phase high performance liquid chromatography and mass spectrum. Their biological activity was detected by E-rose test . Results indicated that the biological activities of the analogues were stronger than standard TP-5 still when their concentrations were 20 times lower than standard TP-5.

关 键 词：穿膜肽 胸腺五肽 固相合成 E-玫瑰花 

分 类 号：O629.71[理学—有机化学]