低温胁迫下棉花子叶蛋白质差异表达的双向电泳分析  

Analysis of two-dimensional electrophoresis for the expressed proteins in cotton cotyledons under cold stress

作　　者：邰付菊[1] 李扬[1] 陈良[1] 李学宝[1] 

机构地区：[1]华中师范大学生命科学学院

出　　处：《华中师范大学学报（自然科学版）》2008年第2期262-266,共5页Journal of Central China Normal University：Natural Sciences

基　　金：国家自然科学基金项目资助(30470930)

摘　　要：采用TCA-丙酮沉淀法,改良优化了棉花蛋白质组研究中的双向电泳技术.通过对根和子叶全细胞蛋白的提取、蛋白的溶解、胶条的选择及电泳等环节的优化,得到了重复性很高、分辨率很好的蛋白质图谱.进一步对5 d苗龄的棉花进行4℃低温处理12 h后,提取子叶的蛋白质,利用双向电泳技术分离全细胞蛋白.并用PDQuest软件分析比较棉花子叶在低温处理下的蛋白表达谱,得到了49个有显著差异的蛋白点.其中,低温处理上调表达的蛋白点有27个,减弱表达的蛋白点有22个,推测这些差异蛋白点可能与低温胁迫相关.The proteins of cotton （Gossypium hirsutum） were extracted by TCA-acetone method and the technique of two-dimensional electrophoresis in cotton proteomics was improved. Some satisfied pictures were obtained through optimizing many key procedures, such as sample preparation, sample dissolution, IPG stripe, IEF electrophoresis, SDS-PAGE. Then proteins from cold treated cotton cotyledon for 12 hours were extracted and separated by 2-DE. A large quantity of protein spots appeared in pictures. PDQuest software was used to analyze the pictures and 49 protein points that have distinct difference in control and treated sample were obtained. 27 proteins were up-regulated, and 22 proteins were down-regulated, suggesting these that proteins were related to cold stress.

关 键 词：棉花 低温处理 双向电泳 

分 类 号：Q946[生物学—植物学]