维吾尔药牛舌草中总黄酮提取工艺的研究  

Study on Extraction Process of Total Flavonoids in Anchusa italica Retiz

作　　者：木合塔尔.奴尔买买提 杨武亮[2] 吐尔洪.艾买尔 倩.纳斯尔 余宝金[2] 买买提.奴尔艾合买提 

机构地区：[1]新疆维吾尔医学高等专科学校,新疆和田848000 [2]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《时珍国医国药》2008年第6期1303-1304,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家重点科学项目-维吾尔医内科学心血管学科的建设项目(No.国中医药发[2004]14号);江西中医学院科研基金课题(No.200605号)

摘　　要：目的研究维吾尔药牛舌草中总黄酮的最佳提取工艺。方法采用正交实验确定各因素的影响大小,确定维吾尔药牛舌草中总黄酮的最佳提取条件,通过紫外分光光度法测定总黄酮含量。结果乙醇浓度对维吾尔药牛舌草中总黄酮的提取影响作用最大,牛舌草中总黄酮的最佳提取工艺为:乙醇75%;料液比1∶6;温度90℃;提取2次,1.5 h/次。结论该研究可为牛舌草总黄酮工业化提取提供理论依据。Objective To optimize the extraction process of total flavonoids in Anchusa italica Retiz. Methods The content of total flavonoids was determined by UV spectrophotometer, The influence of each factor was measured by orthogonal experiments. Resuits The concentration of ethanol had the greatest effect on the extraction of total flavonoids. The optimal condition was as follows：75 % ethanol,90℃, 1：6 （W/V） and extracting 2 times （ 1.5 h each time）. Conclusion This study provides the theory basis for the industrial production.

关 键 词：牛舌草 总黄酮 正交实验 提取工艺 

分 类 号：R291.5[医药卫生—民族医学]