何首乌提取物聚酰胺柱层析流分促血管内皮细胞生长活性研究  

Study on the Promoting Effect on Human Umbilical Vein Vascular Endothelial Cells Proliferation Activity of the Fractions Isolated from Polygonum Multiflorm Thunb Extracted with Polyamide Column Chromatography

作　　者：赵芳[1] 侯华新[1] 黄颖瑜[1] 

机构地区：[1]广西医科大学,广西南宁530021

出　　处：《时珍国医国药》2008年第6期1367-1368,共2页Lishizhen Medicine and Materia Medica Research

基　　金：广西壮族自治区自然基金资助项目(No.桂科能0630006-5E)

摘　　要：目的初步探讨何首乌提取物聚酰胺柱层析流分对人脐静脉血管内皮细胞增殖的影响。方法MTT比色法检测何首乌提取物聚酰胺柱层析流分对人脐静脉血管内皮细胞生长的促进作用;HE染色法观察其流分对人脐静脉血管内皮细胞形态学的影响。结果经聚酰胺柱层析洗脱的何首乌流分对人脐静脉血管内皮细胞增殖具有不同的作用,其中D流分随着浓度的增加,对人脐静脉血管内皮细胞增殖具有促进作用,显微镜下观察到细胞的数量及分裂相增多,B和C流分对细胞的增殖影响不大,而A流分对血管内皮细胞具有抑制生长作用,细胞的数量减少,核固缩。结论何首乌提取物聚酰胺柱层析流分中含有促人脐静脉血管内皮细胞生长的活性物质。Objective To investigate the proliferation effects on human umbilical vein vascular endothelial cells （HUVECs）of the fractions isolated from Polygonum multiflorm Thumb extracted with polyamide column chromatography. Methods The activity of promoting HUVECs growth of each fraction was observed by MTT colorimetric assay. The changes of HUVECs morphology were observed by HE stain. Results The fractions eluted from polyamide column chromatography had different proliferation activities on HUVECs. With the increase of concentration, the fraction D could promote the growth and proliferation of HUVECs significantly. The cells showed morphological change significantly under microscope. Compared with the control, the cell quantity and mitosis of the fraction D were increased. However, there was little or no effects on the proliferation of HUVECs by fraction B and C. In contrast, fraction A had an inhibitory effect on the growth of HUVECs , which caused the decrease in the cell number and karyopycnosis. Conclusion There are some components of promotion growth of HUVECs in the fraction from polygonum multiflorm Thunb.

关 键 词：何首乌 聚酰胺柱色谱 人脐静脉血管内皮细胞 MTT比色法 HE染色法 

分 类 号：R285.5[医药卫生—中药学]