紫丁香叶中总皂苷提取工艺的研究  

Study on the Extraction Process of Total Saponins from Syringa Leaves

作　　者：郝婷婷[1] 严铭铭[2] 郭婷婷[1] 邵帅[2] 张连学[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]长春中医药大学,吉林长春130117

出　　处：《时珍国医国药》2008年第6期1424-1425,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的优选紫丁香叶中总皂苷的提取工艺。方法采用香草醛-冰醋酸-高氯酸显色法,用紫外分光光度计测定不同提取方法下所得紫丁香叶药材中总皂苷的含量,选出紫丁香叶总皂苷的提取工艺。进而设定正交实验,来优选最佳提取工艺。结果总皂苷的最佳提取工艺为70%乙醇回流提取2次,1 h/次,加醇量为每次13倍量。结论此工艺安全、成本低,得到的总皂苷含量高,可作为提取紫丁香叶生产工艺参考。Objective To optimize extraction process of total saponins from Syringa leaves. Methods 5% Vanillin -Acetic Acid Glacial -Perchloric Acid colormetric analysis method and UV were used to measure the content of total saponins from Syringa leaves by different extraction solutions. The orthogonal experiment was used to select the best conditions. Results The best extraction technology was 13 - fold and 70% ethanol, lhour of reflux for 2times of extraction. Conclusion This optimal process is simple, stable and efficient. It is suitable for pharmaceutical production of Syringe leaves.

关 键 词：紫丁香叶 总皂苷 正交设计 提取工艺 

分 类 号：R284.2[医药卫生—中药学]