夏天无高效液相色谱指纹图谱研究  

Study on HPLC Fingerprint of Rhizoma Corydalis Decumbentis

作　　者：戴静波[1] 张佳佳[1] 刘梅[2] 

机构地区：[1]浙江医药高等专科学校,浙江宁波315000 [2]江西中医学院,江西南昌330006

出　　处：《时珍国医国药》2008年第6期1437-1438,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的研究夏天无药材的高效液相色谱(HPLC)指纹图谱,为科学评价及有效控制其质量提供实践方法和参考数据。方法采用Hypersil BDSC18柱,(250 mm×4.6 mm,5μm);以乙腈-水(30∶70,每100 ml中加入pH6.0醋酸/醋酸铵缓冲液3 ml)梯度洗脱,流速1.0 min.min-1;在281 nm波长下检测,测定10批次同一产地夏天无药材,并运用计算机辅助相似性评价系统计算图谱之间的相似度。结果10批夏天无药材指纹图谱之间的相似度很高。结论该方法重现性好,具有一定的专属性,可作为夏天无药材鉴别的依据。Objective To establish HPLC fingerprint of Rhizoma Corydalis decumbentis. Methods HPLC with Hypersil BDS column was used with mixture of acetonitrile and water as mobile phase in a gradient elution. The flow rate was 1.0 ml · min ^-1. The detection wavelength was at 281nm . Ten batches of Rhizoma Corydalis Decumbentis from the same producing areas were analyzed to establish a fingerprint . The similarity in fingerprint was calculated by computer - aided similarity appraising system. Results All of the 10 batches of samples share a high similarity. Conclusion The method is reproducible and exclusive. It may provide the basis for identifying Xiatianwu.

关 键 词：夏天无 高效液相色谱 相似度 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]