广西田七总黄酮的超声波提取工艺及含量测定  

Extraction Technique by Ultrasonic Wave and Content Determination of the Total Flavone from TianQi in Guangxi

作　　者：杨海菊[1] 孔德鑫[1] 黄荣韶[1] 郑钰艳[1] 秦志长[1] 黄万清 

机构地区：[1]广西大学农学院,广西南宁530005 [2]广西靖西县农工业局,广西靖西533800

出　　处：《抗感染药学》2008年第2期88-91,共4页Anti-infection Pharmacy

基　　金：广西科技攻关计划项目(编号:063000Z-3A)

摘　　要：目的:以总黄酮为指标,优化超声波提取田七黄酮的最佳工艺条件,测定广西田七不同部位黄酮含量。方法:运用正交设计筛选超声提取参数,以芦丁为对照品,采用NaNO_2-Al(NO_3)_3-NaOH比色法测定总黄酮含量。结果:采用φ(C_2H_6O)=70%为溶剂,预先浸泡2 h,超声波提取3次,每次40 min,以固液比为1:20为最佳超声提取工艺。并测定田七中不同部位黄酮含量,分别为根茎1.394 mg/g,主根0.646 mg/g,侧根0.785 mg/g,须根0.982 mg/g。结论:超声波提取工艺简便,方法可行;不同部位黄酮含量差异较大,根茎含量最高,侧根和须根其次,而主根含量最低。Objective： To establish the optimized process of the total flavone extraction in Panax notoginseng （Tianqi） by ultrasonic wave technique, and to determinate the total flavone content. Methods： The extraction conditions with ultrasonic wave were studied by orthogonal design and the content of flavone was determined by the NaNO2- Al（NO3） 3-NaOH colorimetric method. Results： The optimique process of the technique was： the ratio of[S]/[L] 1 ： 20, the extraction time 40 min and extracted 3 times with φ（C2H6O） = 70%. The flavone content of rhizome, taproot, secondary root and fibre root was 1.394 mg/g, 0.646 mg/g, 0.785 mg/g and 0.982 mg/g respectively. Conclusion： the ultrasonic wave extraction technique of flavone is convenient and the content of the total flavone in Panax notoginseng in Guangxi province was different in different parts： rhizome was the highest and taproot was the lowest.

关 键 词：田七 总黄酮 超声提取 含量测定 

分 类 号：R931.71[医药卫生—生药学] R927.2[医药卫生—药学]