小麦在铜、镉胁迫下体内含巯基物质对解毒机制的研究  

The Study on Sulfhydryl Compound to the Mechanism of Detoxification in Wheat Under Copper and Cadmium Stress

作　　者：王海鸥[1] 钟广蓉[1] 刘晓峰[1] 弓爱君[1] 李晓晶[1] 

机构地区：[1]北京科技大学生物科学与技术系,北京100083

出　　处：《华北农学报》2008年第3期158-161,共4页Acta Agriculturae Boreali-Sinica

基　　金：北京科技大学校基金(00009184)

摘　　要：将小麦幼苗分别放在含有100μmol／L Cu2＋的培养液和100μmol／LCd2＋的培养液中培养，观察其体内重金属含量、丙二醛（MDA）、植物螯合肽（Pc）和谷胱甘肽（GSH）的变化。试验结果表明，在铜、镉胁迫下，小麦体内重金属含量逐渐增加，尤以根中增加明显，约75倍，而叶中增加较少，为2～5倍；无论是在根中还是在叶中，镉的含量都高于铜的含量；铜、镉胁迫下小麦叶片膜脂过氧化程度都高于根部无论是铜胁迫还是镉胁迫下，小麦体内叶片的GSH含量均高于根部，而PC含量均低于根部。Wheat were cultivated in hydroponic culture solution with 100 μmol/L Cu2 ＋ and 100 μmol/L Cd2 ＋ separately, the concentration of copper and cadmium, malonaldehyde, phytochelatin and glutathione in roots and leaf were investigated. The results revealed that under stress the concentration of copper and cadmium increased gradually, especially in roots the concentration increased remarkably, 75 fold growth approximately, but in leaf the increase was slowly, 2 - 5 fold approximately. The content of cadmium is higher than copper in leaf and roots. Lipid peroxidation in leaf was stronger than that in roots. Under heavy metal stress the concentration of glutathione in leaf was higher than that in roots, while phytochelatins in leaf was lower than that in roots.

关 键 词：小麦 镉 铜 丙二醛 植物螯合肽 谷胱甘肽 

分 类 号：Q945[生物学—植物学]