大鼠骨骼肌钝挫伤分级模型的建立  

Animal model of grading skeletal muscle contusion due to blunt impact in rats

作　　者：于天水[1] 官大威[1] 程子惠[1] 赵锐[1] 胡更奕[1] 朱瑞霞[2] 王玲[1] 郭晓冲[1] 王昌亮[1] 

机构地区：[1]中国医科大学法医学院法医病理学教研室,辽宁沈阳110001 [2]中国医科大学第一临床学院神经内科,辽宁沈阳110001

出　　处：《法医学杂志》2008年第3期168-171,241,共5页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(30271347);教育部留学归国基金资助项目(教外留司[2000]367)

摘　　要：目的建立一个控制性、重复性好,并可分级的骨骼肌挫伤动物模型。方法采用自行设计的机械式撞击装置,以撞击速度(velocity,v)和形变(deformation,DF)作为外力参数,并保持较恒定的撞击时间,使击锤打击瞬间速度不同,造成轻、中、重不同程度的大鼠腓肠肌挫伤。结果在肉眼、HE染色观察下,39只大鼠腓肠肌出现可分级的病理学改变。在低撞击水平(v:2m/s,DF:5.5mm),轻度挫伤组病理改变局限于大鼠腓肠肌肌外膜和腓肠肌表层部位;在中度撞击水平(v:2.5m/s,DF:6.5mm),中度挫伤组病理改变可见于大鼠腓肠肌肌外膜和大部分腓肠肌;在高撞击水平(v:3m/s,DF:7.5mm),重度挫伤组病理改变除中度挫伤组累及部位外,还伤及比目鱼肌。结论该模型制作出常见的钝力性骨骼肌挫伤,以撞击速度和形变作为外力参数,成功建立了机械骨骼肌挫伤分级模型,致伤程度较一致,是骨骼肌挫伤研究较合适的动物模型,为深入研究钝力性骨骼肌挫伤奠定了基础。Objective To establish a new animal be controllable and repetitive. Methods The rats model of grading skeletal muscle contusions that could gastrocnemius was injured by a new weight-dropping device designed. The force acting on gastrocnemius with a comparatively constant duration and inducing elastic deformation of the gastrocnemius was expressed with velocity （v） and deformation （DF）. Instant velocity was changed to create gastrocnemius contusions. Pathological changes of gastrocnemius were graded by the gross and histological examinations of 39 rats. Results At low level of impact （v： 2 m/s, DF： 5.5 mm）, mild injuries were detected in epimysium and superficial layer of gastrocnemius. At moderate level of impact （v： 2.5 m/s, DF： 6.5mm）, the injuries were observed in epimysium and whole gastrocnemius. At high level of impact （v： 3 m/s, DF： 7.5 mm）, severe injuries were seen deeper to soleus with more extensive skeletal muscle damage. Conclusion Grading of skeletal muscle blunt force contusion is created by parameter of velocity and muscle deformation. The model could be used for further research on skeletal muscle contusions.

关 键 词：法医病理学 骨骼肌挫伤 实验性动物模型 大鼠 

分 类 号：R873[医药卫生—运动医学] R782.4[医药卫生—临床医学]