湖北道地药材北柴胡及醋制北柴胡的指纹图谱研究  

Studies on Fingerprints of Bupleurum chinense and Vinegar-bakedBupleurum chinense from Hubei Province

作　　者：聂飒[1] 刘小平[1] 陈绍文[2] 李凯[2] 

机构地区：[1]武汉理工大学化学工程学院,湖北武汉430070 [2]湖北中医学院,湖北武汉430061

出　　处：《中药材》2008年第5期657-659,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:建立湖北省保康GAP产地净柴胡、醋柴胡指纹图谱并探讨炮制前后的变化。方法:采用HPLC法。Hypersil ODS(250 mm×4.6 mm,5μm)色谱柱;流动相:乙腈-水梯度洗脱;流速:1.0 ml/min;以紫外检测器(UV)检测,检测条件:检测波长203 nm;柱温25℃。结果:净柴胡和醋柴胡相似度均大于0.95,归纳出净柴胡13个特征指纹峰,醋柴胡15个特征指纹峰。结论:用HPLC法建立了湖北道地药材GAP示范基地的净柴胡和醋柴胡的指纹图谱,并初步研究了二者之间差别。Objective：To establish the HPLC Fingerprint of Bupleurum chinense from GAP provenance of Hubei province Baokang,and discuss the changes before and after processing.Methods：Using HPLC detection method equipped with Hypersil ODS column（5 μm,4.6 mm×250 mm）,using acetonitrile-water as mobile phase gradient elution,flow as 1.0 ml/min,UV detector set at 203 nm,column temperature was at 25℃.Results：Similarity of processed Bupleurum chinense and Bupleurum chinense exceeded 0.95,and there were 13 characteristic fingerprints of processed Bupleurum chinense,15 characteristic fingerprints of Bupleurum chinense.Conclusion：The HPLC Fingerprint of Bupleurum chinense from GAP provenance of Hubei province Baokang are established,and the difference between Bupleurum chinense before and after processing is studied initially.

关 键 词：柴胡 指纹图谱 高效液相色谱法 

分 类 号：R282.5[医药卫生—中药学]