TLCS法对广西产喜树果中喜树碱含量动态积累的研究  

The Study of Dynamic Acccumulation of Camptothecin in Camptotheca acuminata Fruit by TLCS

作　　者：蒋明廉[1] 黄瑞松[2] 吴毅 周芳礼[3] 苏青[2] 韦爱艳[4] 

机构地区：[1]广西南宁食品药品检验所,广西南宁530001 [2]广西民族医药研究所,广西南宁530001 [3]广西大学化学化工学院,广西南宁530004 [4]广西中医学院,广西南宁530001

出　　处：《中药材》2008年第5期684-686,共3页Journal of Chinese Medicinal Materials

基　　金：广西自然科学基金项目(桂科自0542055);广西大型仪器协作共用网资助项目(327-2006-016)

摘　　要：目的：建立喜树果药材中喜树碱含量的TLCS测定方法,并对喜树果药材中喜树碱含量的动态积累进行分析,确定喜树果的最佳采收期。方法：采用硅胶H-CMC-Na薄层板,展开剂为氯仿-丙酮（7∶3）,单波长线性扫描,λs=360 nm。结果：喜树碱在0.0542～0.3252μg范围内与峰面积积分值呈良好的线性关系,平均回收率为97.13%,RSD=1.76%;6个产地不同时间采集喜树果药材喜树碱含量的动态积累情况相似,均为8～9月份喜树碱含量较高。结论：该方法简便、准确、可靠,可作为喜树果中喜树碱含量的测定方法;广西产喜树果最佳采收期应为每年的8～9月份。Objective：To establish TLC scanning method for the determination of camptothecin in Camptotheca acuminata fruit,and analysis the dynamic accumulation of camptothecin in Camptotheca acuminata fruit to find out the best collection period.Methods：Silica gel H-CMC-Na thin layer plate was adopted in the determination with chloroform-acetone（7∶3）used as deeloper,Single-wavelength and linear scanning of TLC was used,and the detection wavelength was 360 nm.Results：There was a good linear relationship for Comptothecin within the range of 0.0542～0.3252 μg,the average recovery was 97.13%,RSD was 1.76%.Conclusion：The method is accurate,simple and reliable,and can be used for the determination of camptothecin in Camptotheca acuminata fruit and dynamic accumulation research.

关 键 词：喜树果 喜树碱 动态积累 薄层扫描法 

分 类 号：R284.1[医药卫生—中药学]