紫草多糖的分离纯化及生物活性研究  

Isolation,Purification and Biological Activities of Arnebia euchroma Glycosaminoglycans

作　　者：邓远辉 王海兰[2] 韩凌 

机构地区：[1]广州中医药大学第二附属医院,广东广州510120 [2]广州中医药大学,广东广州510405

出　　处：《中药材》2008年第5期753-756,共4页Journal of Chinese Medicinal Materials

基　　金：广东省医学科研基金资助项目(A2003260)

摘　　要：目的:研究紫草多糖的提取、分离与纯化方法,并初步探讨其生物活性。方法:采用蒸馏水提取、乙醇分级沉淀、Sephadex凝胶柱色谱分离、纯化获得紫草多糖,由HPLC确定其相对分子质量和质量分数;建立荧光定量PCR检测方法,将实验分为生理盐水组和8个不同剂量紫草多糖组,观察与疣体组织作用后,抗HPV效果。结果:经HPLC检测,该组分为两个分子量的多糖,其重均分子量分别为27336、1152。生物活性检测结果表明,紫草粗多糖具有体外抑制HPV-DNA活性,最低有效浓度为0.781mg/ml。结论:本方法提取的紫草多糖为含两个分子量的多糖混合物,具有明显的抗人乳头瘤病毒作用。Objective：To study the methods of extraction,isolation,purification and biological activities of Arnebia euchroma glycosaminoglycans（AEG）.Methods：AEG was purified by distilled water extraction,ethanol fractionation,Sephadex column chromatography.The purity and molecular weight and concentration of AEG were measured by HPLC;We divided the experiment into Physiological Saline group and the other eight groups with different doses of AEG,established RT-PRC method to observe the anti-HPV effect after their actions on the verruca tissues.Results：Using HPLC,the group of AEG was divided into two glycosaminoglycans with different molecular weight as 27336 and 1152.Bioassay results showed that AEG had anti-HPV-DNA activity,the lowest effective concentration was 0.781mg/ml.Conclusion：AEG extracted by this method is a mixture with two molecular weight glycosaminoglycans,and has obvious anti-HPV effect.

关 键 词：紫草多糖 提取 分离 HPV-DNA 

分 类 号：R284.2[医药卫生—中药学] R285.5[医药卫生—中医学]