酸-碱滴定法测定海洋浮游植物细胞表面积  

DETERMINING SURFACE AREAS OF MARINE PHYTOPLANKTON CELLS BY ACID-BASE TITRATION METHOD

作　　者：王修林[1] 马延军[1] 李继亮[1] 郁纬军 孙汉章[1] 

机构地区：[1]青岛海洋大学,青岛266003

出　　处：《海洋与湖沼》1997年第4期356-362,共7页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金!49376271

摘　　要：1995年改进了浮游植物表面积的酸碱滴定测定法，其中应用改进BET方程对酸滴定曲线拟合求最大表面酸量，由此测定了18种海洋浮游植物（包括8种绿藻，7种硅藻，2种甲藻，1种红藻）的细胞表面积。结果表明，该方法精密度为15．2％，所测定的18种海洋浮游植物的表面积为2．78×10-8—6．9×10-6m2，变异系数为0．9％—31．3％，较传统的用光／电子显微镜粗略估算的方法，更为精确，并且设备简单，操作方便。In 1995, the acid-base titration method for determining the surface areaof marine phytoplankton cells was improved, in which the modified BET equation wasused to estimate the maximum surface acid amount {M+ }. by fitting the surfase acidamount {MOH2+}. to [H+]. Estimate of {M+ } T with the modified BET equation ismore feasible and reliable than measurement by the back-titration technique liable tounder or overestimate of {M+ }T The acid-base titration technique to measure {MOH2+}of phytoplankton cells is based on the assumption that the hydrous cell walls havecarbohydrates or other structura compounds which can behave as surface Bronstedacid-base sites due to coordination of environmenta H2O molecules. The improvedmethod was applied to 18 diverse phytoplanktons species (including 7 diatoms, 2flagellates, 8 green phytoplanktone and 1 red phytoplankton) maintained in seawatorcultures.For the species exaxnined, the surface area of individual cell (A) ranged from2.78 ×10-8m2 for Nannochloropsis oculata to 690 ×10-8m2 for Dunaliella viridis.Measurement accuracy was 15.2%(n = 6). A values of marine phytoplankton mayrelate to, but is not dominated by, the cell size. Furthermore, A values wereapproximarely 2 to 4 orders of magnitude more than the geometric surface area of thecell, assuming that the phytoplankton cell is a smooth ball. This suggested that marinephytoplankton A determined by the asid-base titration method may reveal the actualsurface area of marine phytoplankton cells in seawater. Preliminary study showed thatthe improved method may be more promising and accurate than light/ electronmicroscopic measurements for coarse estimation of the surface areas of phytoplanktonsonly if the cell walls behave like a Bronsted acid-base site due to the coordination ofenvironmntal H2O molecules. In addition, the method has merits, such as use ofsimple equlpment convenient operahon, and easy dissemination. It is useful for studyon the quantitative relationship between bioconcentration of HOC in marinephytoplanktone and th

关 键 词：海洋 浮游植物 表面积 酸碱滴定 细胞 

分 类 号：Q948.885.3[生物学—植物学]