高＋Gx过载对猴颌下腺组织结构与c-jun基因表达的影响  

Effect of high ＋Gx load on structure and c-jun expression of submandibular gland in rhesus macaque

作　　者：汤楚华[1] 张建中[1] 牛忠英[1] 施生根[1] 李冬[1] 

机构地区：[1]解放军第三○六医院,北京100101

出　　处：《中华航空航天医学杂志》2007年第1期37-39,F0004,共4页Chinese Journal of Aerospace Medicine

摘　　要：目的观察高＋Gx过载作用对猴颌下腺组织结构和c—jun基因表达的影响。方法9只雄性恒河猴，随机分为4组。对照组与实验1、2、3组受＋Gx及其作用时间分别为1Gx／300s、＋15Gx／200s、＋18Gx／165s、＋21Gx／140s。使用专用动物离心机，加载后的动物即刻处死、取材、制片、染色。用免疫组织化学方法观察猴颌下腺组织c—jun表达。结果组织病理学观察：对照组和实验组猴颌下腺组织均未见明显病理学改变。免疫组化观察：对照组颌下腺导管上皮细胞c—jun为弱阳性表达；实验组c—jun表达明显增强，阳性产物呈棕黄色颗粒状弥漫分布于颌下腺导管上皮细胞核，作用强度不同的＋Gx组之间无明显差别。结论高＋Gx环境中可引起猴颌下腺导管上皮细胞c—jun基因表达增强。Objective To observe the structural changes and c jun expression of submandibular gland in rhesusmacaqnl followimg high sustained ＋Gx loads. Methods Nine male rhesus macaques were randomly divided into four groups： control group underwent ＋1 Gx/300 s on animal centrifuge, and experimental groups 1, 2 and 3 underwent ＋15 Gx/200 s, ＋18 Gx/165 s and ＋21 G x/140 s respectively. The tissue of submandibular gland was fixed with 10% buffered formaldehyde and embed ded with paraffin, and histopathological changes were observed with microscope. The expression of c- jun in submandibular gland was detected by immunohistochemical staining （S-P method） Results gross inspection and histological observation showed that no significant pathological changes were ob served in the submandibular gland tissues in all 3 experimental groups of the animal. Immunohistochemical staining demonstrated that over-expression of c-jun was detected in the nuclei of the ductal cells in 3 experimental groups, and negative or slight positive staining was also observed in the ductal epithelium of the gland in control animals. There was no obvious difference of c-jun expression in different experimental groups. Conclusions High sustained ＋Gx can induce the over-expression of c jun in the submandibular gland of the experimental monkeys.

关 键 词：超重力 颌下腺 基因表达 免疫组织化学 恒河猴 

分 类 号：R686[医药卫生—骨科学]