稻瘟菌品种特异性改变突变体筛选及其遗传分析  

Screening and genetic analysis of the cultivar specificity altered mutant of Magnaporthe grisea

作　　者：魏士平[1] 韦建福[1] 彭友良[1] 

机构地区：[1]中国农业大学植物病理系

出　　处：《河北农业大学学报》2007年第1期71-75,共5页Journal of Hebei Agricultural University

基　　金：国家"973"项目(G2000016201);国家"863"项目(2001AA223081)

摘　　要：通过筛选稻瘟菌(Magnaporthe grisea)P131小种的REMI(Restriction enzyme mediated integration)转化体库,获得1株对水稻品种爱知旭品种特异性改变的突变体MS220。Southern及质粒拯救分析表明:外源质粒在基因组中的整合为单位点、双拷贝反向串联插入。以质粒拯救所获得的侧端序列为探针,通过对爱知旭的毒性菌株和无毒菌株在插入位点处的RFLP分析,结果表明:两者在SacI酶切的泳道内出现了明显的多态性,由此推测,控制野生型菌株P131的无毒相关基因,由于外源质粒插入而发生突变。因此,可以以此为标记,克隆控制该表型的基因。MS220,a mutant altered its cultivar specific pathogenicity on Aichiasahi（carrying resistance gene Pi-a）,was screened out from the pool of transformants generated by Restriction Enzyme Mediated Integration（REMI）.Genomic Southern blot and plasmid rescue analyses showed that the plasmid was inserted into the genome of MS220 with single site,two copies by invert repeat.As the flanking sequence of rescued plasmid was a probe,RFLP analysis of the polymorphism at the locus of the plasmid inserted between virulent and avirulent strains on Aichiasahi,the result showed that the polymorphism was clearly appeared in the lanes with Sac I digested genomic DNA.It implicated that the avirulent-related locus was tagged by the plasmid insertion in MS220.So,this tagged gene can be cloned as the inserted plasmid was a marker.

关 键 词：稻瘟菌 无毒相关基因 插入突变 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]