玫瑰黄链霉菌Men-myco-93-63有效成分的初步提取与纯化  

The primary characterization of the efficiency component from Streptomyces roseoflavus Men-myco-93-63

作　　者：赵志泉[1] 赤国彤 孟庆芳[1] 杨文香[1] 张汀[1] 刘大群[1] 

机构地区：[1]河北农业大学植物保护学院河北省农作物病虫害生物防治工程技术研究中心,河北保定071001

出　　处：《河北农业大学学报》2007年第1期76-78,104,共4页Journal of Hebei Agricultural University

基　　金：河北自然科学基金(301163);国家863计划基金(2003AA241130)

摘　　要：为了解玫瑰黄链霉菌Men-myco-93-63的代谢产物和其生物活性,对该菌株进行摇瓶培养,从菌丝中提取到一种对棉花黄萎病菌有明显抑制作用的有效成分。经过XAD-1600型大孔吸附树脂,ODS C18硅胶中压柱分离得到黄白色粉末。HPLC检测后,初步断定该物质是一类非常相似的物质组成的混合物,并且极有可能具备互变异构的特性。In order to find out the metabolism substance and its bioactivity of Streptomyces roseoflavus Men-myco-93-63,medium which can enrich the hyphae was used to isolate a group componets which have a distictive inhibition against the Verticillium dahliae.Light yellow powder was obtained after using the macropore gel XAD-1600 and the ODS C18 colunm for separation. It was initially concluded that it is composed of a series of homologous components,which were very likely dynamic isomers after analyzed by HPLC.

关 键 词：玫瑰黄链霉菌 有效成分 分离纯化 

分 类 号：S852.619[农业科学—基础兽医学]