红叶石楠离体快繁技术体系的建立与优化  

Establishment and optimization Photinia frasery rapid propagation system

作　　者：侯春燕[1] 任丽梅[1] 张洁[1] 王冬梅[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001

出　　处：《河北农业大学学报》2007年第5期44-47,共4页Journal of Hebei Agricultural University

基　　金：河北农业大学校青年基金2004

摘　　要：以红叶石楠茎尖为外植体,探讨不同植物激素6-BA、IBA、NAA的浓度对试管苗增殖以及植株再生的影响,建立了一套红叶石楠离体培养技术体系。试验结果表明:红叶石楠初代培养采用MS+6-BA1.0 mg/L+NAA 0.1 mg/L培养基、茎尖继代培养采用MS+6-BA 2.0 mg/L和NAA 0.1 mg/L的培养基为宜,通过正交试验确定,植株增高选用MS+6-BA1.0+IBA 0.2 mg/L+NAA 0.2 mg/L的培养基效果较好,生根培养选用1/2MS+IBA1.0～1.5 mg/L培养基,生根率、移栽成活率可达100%。Using the stem segment with single bud as the explant, this paper probes into the effects of different concentration 6 - BA, NAA, IBA on proliferation and plantlet regeneration of Photinia frasery. The results showed that the appropriate medium for bud induction is MS ＋ 6 - BA 1.0 mg/L ＋ NAA 0.1 rng/L,the medium of multiplication is MS ＋ 6 - BA 2.0 mg/L ＋ NAA 0.1 mg/L. Using orthogonal tests to determine the medium for plant grow is MS ＋ 6 - BA 1.0 mg/L ＋ IBA 0.2 mg/L ＋NAA 0.2 mg/L,and the medium of rooting is 1/2 MS ＋ IBA 1.0-1.5 mg/L,with up to 100% survival rate.

关 键 词：红叶石楠 组织培养 正交试验 快速繁殖 

分 类 号：S336[农业科学—作物遗传育种]