腐烂茎线虫不同地理种群ITS区序列比对及系统发育  

Alignments of rDNA-ITS sequences and phylogeny of different geo-populations of Ditylenchus destructor in China

作　　者：王金成[1] 季镭[2] 黄国明[1] 杨秀丽[1] 林茂松[2] 

机构地区：[1]天津出入境检验检疫局,天津300456 [2]南京农业大学植病系,江苏南京210095

出　　处：《河北农业大学学报》2007年第5期79-83,98,共6页Journal of Hebei Agricultural University

基　　金：天津自然科学基金资助项目(05YFJMJC10800)

摘　　要：为弄清我国腐烂茎线虫(Ditylenchus destructor)不同地理种群ITS(Internal transcribed spacer)区之间的碱基差异及系统发育关系,本研究通过网络软件Clustalw1.83对腐烂茎线虫的核糖体ITS区核酸序列进行了比对,结果发现腐烂茎线虫的8个种群中,DEll、DEly、DEAY987007三个种群(I组)内部之间的碱基差异在1%以内,其它的5个种群(II组)之间的差异为0～1%,而I组与II组种群之间的差异达到了15%(根据ITS1+5.8s+ITS2序列)或20%(根据ITS1+ITS2序列)。这说明我国腐烂茎线虫很可能是1个复合种。根据ITS区核酸序列构建的系统发育图同样显示,我国腐烂茎线虫的7个地理种群明显分为2个分支A和B。The ITS sequences of 8 populations of sweet potato, stem nematode, Ditylenchus destructor, were analyzed. The pair- wise divergences for ITS1 ＋ ITS2 sequences and ITS1 ＋ 5.8s ＋ ITS2 sequences of 3 populations （group Ⅰ）, DEll, DEly and DEAY987007, are within 1%. The pair- wise divergence within other 5 populations （group Ⅱ）, DEjl, DEsg, DEws, DEwy, DEhblf, varied from 0-1%. However, the divergence between group Ⅰ and group Ⅱ is 15% for ITSI ＋ 5.8s ＋ ITS2 sequences or 20 % for ITS1 ＋ ITS2 sequences. The MP analysis divided the 8 abovementioned populations into two clades. The clade B includes DEll, DEly and DEAY987007 （group Ⅰ）, and the other 5 populations in group Ⅱ belong to clade A.

关 键 词：腐烂茎线虫 鳞球茎茎线虫 食菌茎线虫 ITS(Internal transcribed spacer) 系统发育关系 

分 类 号：S435.621.29[农业科学—农业昆虫与害虫防治]