鸡新城疫强度株的分离鉴定与免疫保护试验  

Isolation and identification of high virulent strains of NDV and immune protection of newcastle disease virus

作　　者：王玉珠[1] 李庆锁[2] 李占雷[1] 刘新云[1] 孙继国[1] 刘虹[2] 王爱华[3] 

机构地区：[1]河北农业大学,河北保定071001 [2]河北省畜牧兽医研究所,河北保定071000 [3]河北北方学院动物医学系,河北宣化075131

出　　处：《河北农业大学学报》2007年第3期75-78,共4页Journal of Hebei Agricultural University

摘　　要：从河北省保定地区发病鸡群中分离到2株有血凝性的分离物,其血凝作用可被NDV阳性血清所抑制,而不能被AIV(H9亚型)、EDS 76所抑制,表明2株分离物均为新城疫病毒。对2株NDV的毒力指数(MDT、IC-PI、IVPI)测定结果显示:MDT分别为40.8 h和53.7 h,ICPI分别为1.96和1.86,IVPI分别为2.89和2.72,表明2株NDV均为新城疫强毒株。采用分离的HBW株制成油乳剂灭活疫苗,分别以HBW株和LaSota株的灭活苗免疫试验鸡,进行疫苗免疫效果比较和交叉免疫保护试验。结果显示:(1)分离株灭活苗免疫组鸡各个时期所产生的HI抗体效价和Lasota灭活苗免疫组鸡的抗体效价相差不明显,2组的升降规律也基本一致。(2)分离株灭活苗免疫组鸡(HI抗体效价为6log2以上时)对相同剂量F48E9标准毒株和2个分离株的攻击可给予100%的保护,Lasota灭活苗免疫组鸡(HI抗体效价为6log2以上时)对相同剂量F48E9标准毒株和2个分离株的攻击不能给予100%的保护。说明NDV不同基因型毒株间的毒力和抗原性有一定的差异。Two isolates having haemagglution property have been isolated from the disease-infected chicken house in Baoding of Hebei Province, the haemagglution property of the two isolates can be inhibited by the positive antiserum of NDV, not by the positive serum of AIV（H9 subtype）, and EDS76, which shows that the two strains are NDV. The result of pathogenicity tests （MDT, ICPI, IVPI） show that the two NDV isolates claim these data： MDT,40.8 h and 53.7 h; ICPI, 1.96 and 1.86; IVPI,2.89 and 2.72, which indicates that the two NDV isolates are highly virulent. Inactivated oil-emulsion vaccine was made by isolated strain of HBW. The efficacy were compared between inactivated LaSota vaccine and isolates inactivated vaccine and the experiment of cross-protection of vaccine assess by immunized chickens with isolate inactivated and LaSota inactivated vaccine.Result showed that （1） There is no apparent difference of HI antibody between chickens inoculated with isolates inactivated vaccine and inactivated LaSota vaccine during all experiment period; （2） All chickens with higher than 61og2 HI titer inoculated with isolates inactivated vaccine could obtain 100 % protection against F48E9 and two isolated strains, while all chickens with higher than 61og2 HI titer inoculated with LaSota inactivated vaccine could not obtain 100% protection against F48E9 and two isolated strains. Result showed that variation of pathogenicity and antigenicity exist among the strains of NDV of different genotypes.

关 键 词：新城疫病毒 分离 鉴定 交叉免疫保护 

分 类 号：S585.31[农业科学—作物学]